Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Mutational analysis of chicken interleukin 2
Autore:
Kolodsick, JE; Stepaniak, JA; Hu, WP; Sundick, RS;
Indirizzi:
Wayne State Univ, Sch Med, Dept Immunol & Microbiol, Detroit, MI 48201 USAWayne State Univ Detroit MI USA 48201 & Microbiol, Detroit, MI 48201 USA
Titolo Testata:
CYTOKINE
fascicolo: 6, volume: 13, anno: 2001,
pagine: 317 - 324
SICI:
1043-4666(20010321)13:6<317:MAOCI2>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE INTERLEUKIN-2; RECEPTOR-BINDING; PROLIFERATIVE ACTIVITY; BIOLOGICAL-ACTIVITY; RESIDUES; PROTEINS; SITE; IL-2; IDENTIFICATION; ANTAGONIST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Sundick, RS Wayne State Univ, Sch Med, Dept Immunol & Microbiol, 540 E Canfield,117 Lande, Detroit, MI 48201 USA Wayne State Univ 540 E Canfield,117 Lande Detroit MI USA 48201
Citazione:
J.E. Kolodsick et al., "Mutational analysis of chicken interleukin 2", CYTOKINE, 13(6), 2001, pp. 317-324

Abstract

Chicken interleukin 2 (chlL-2) has low, but significant, homology to both mammalian IL-2 and mammalian IL-15, In vie,v of its unique phylogenetic position and potential use as a vaccine adjuvant, a detailed mutational analysis for critical functional sites was undertaken. It was found that Asp17 isa critical N terminal contact site for binding to the putative chIL-2 receptor, which is similar to results obtained for mammalian IL-2 and IL-15, Analysis of the C terminus did not reveal a single critical amino acid. However, deletion mutant studies demonstrated that removal of C terminal amino acids yielded proteins with decreased bioactivity and that this decrease wasa function of the number and kind of amino acids removed. This study is the first nonmammalian IL-2 mutational analysis and proposes a model for the interaction between chIL-2 and its receptor. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 15:28:57