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Titolo:
Cationic liposomes and nucleic acids
Autore:
Woodle, MC; Scaria, P;
Indirizzi:
Intradigm Corp, Bethesda, MD 20817 USA Intradigm Corp Bethesda MD USA 20817 tradigm Corp, Bethesda, MD 20817 USA
Titolo Testata:
CURRENT OPINION IN COLLOID & INTERFACE SCIENCE
fascicolo: 1, volume: 6, anno: 2001,
pagine: 78 - 84
SICI:
1359-0294(200102)6:1<78:CLANA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-TRANSFER; PLASMID-LIPID PARTICLES; DNA COMPLEXES; IN-VIVO; INTRACELLULAR TRAFFICKING; TRANSGENE EXPRESSION; CELLULAR-IMMUNITY; DELIVERY; CELLS; THERAPY;
Keywords:
gene therapy; lipoplex; liposome; cationic lipid; DNA delivery; colloid; oligonucleotide;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Woodle, MC Intradigm Corp, Bethesda, MD 20817 USA Intradigm Corp BethesdaMD USA 20817 p, Bethesda, MD 20817 USA
Citazione:
M.C. Woodle e P. Scaria, "Cationic liposomes and nucleic acids", CURR OP COL, 6(1), 2001, pp. 78-84

Abstract

During the past decade, cationic lipids have emerged as the primary choicefor gene delivery in vitro, i.e. transfection of cultured cells. A number of lipids with cationic head groups have been synthesized and evaluated. However, their success in vivo for gene therapy has been limited. To date, simple electrostatic complexes of cationic lipid mixtures with DNA have been hampered in numerous aspects: lack of colloidal stability, relatively low efficiency observed as expression levels or % of transfected cells, short duration of expression, and most importantly, non-specific interactions with many cells and tissues. Appreciation of the complexity of in vivo requirements and especially opposing requirements, for extra- and intracellular trafficking, is leading to engineered designs of gene delivery vectors containing cationic lipids. These designs attempt to assemble layered colloidal systems that accommodate the multiple functions required to traverse the various extra- and intracellular barriers. Successful development of such systems will depend on the ability to characterize and optimize each step rather than rely only on reporter gene expression, in addition to the obvious needto characterize the layered nature of the complexes. Importantly, many pharmacological aspects must be considered, especially control of the biodistribution and toxicity. Initial reports on such systems appear to provide at least a proof of the concept. (C) 2001 Published by Elsevier Science Ltd. All rights reserved.

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Documento generato il 07/07/20 alle ore 22:24:45