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Titolo:
Shotgun phage display cloning
Autore:
Jacobsson, K; Frykberg, L;
Indirizzi:
Swedish Univ Agr Sci, Dept Microbiol, SE-75007 Uppsala, Sweden Swedish Univ Agr Sci Uppsala Sweden SE-75007 l, SE-75007 Uppsala, Sweden
Titolo Testata:
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
fascicolo: 2, volume: 4, anno: 2001,
pagine: 135 - 143
SICI:
1386-2073(200104)4:2<135:SPDC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBRONECTIN-BINDING PROTEIN; STAPHYLOCOCCUS-AUREUS; NUCLEOTIDE-SEQUENCE; FILAMENTOUS PHAGE; GENE; SURFACE; EXPRESSION; SELECTION; FIBRINOGEN; ANTIBODIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Jacobsson, K Swedish Univ Agr Sci, Dept Microbiol, Box 705, SE-75007 Uppsala, Sweden Swedish Univ Agr Sci Box 705 Uppsala Sweden SE-75007 Sweden
Citazione:
K. Jacobsson e L. Frykberg, "Shotgun phage display cloning", COMB CHEM H, 4(2), 2001, pp. 135-143

Abstract

Shotgun phage display cloning is a useful tool for studying interactions between bacterial and host proteins. Libraries are constructed by cloning randomly fragmented prokaryotic DNA into phagemid-vectors. Theoretically, these libraries will consist of phages that together display all proteins encoded by the bacterial genome. Selecting a gene III-based library, made from Staphylococcus aureus DNA, against IgG and fibronectin resulted in 20-40% positive clones after two pannings. Increasing the number of fusion proteinsper phage particle by using gene VIII-based display, increased the frequency of correct clones to 75-100%.

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Documento generato il 04/04/20 alle ore 21:48:59