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Titolo:
Modified Granada Agar Medium for the detection of group B streptococcus carriage in pregnant women
Autore:
Claeys, G; Verschraegen, G; Temmerman, M;
Indirizzi:
State Univ Ghent, Dept Microbiol, Microbiol Lab, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 crobiol Lab, B-9000 Ghent, Belgium State Univ Ghent, Dept Obstet & Gynecol, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 t & Gynecol, B-9000 Ghent, Belgium
Titolo Testata:
CLINICAL MICROBIOLOGY AND INFECTION
fascicolo: 1, volume: 7, anno: 2001,
pagine: 22 - 24
SICI:
1198-743X(200101)7:1<22:MGAMFT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Keywords:
GBS; group B streptococcus; Streptococcus agalactiae; early-onset sepsis; prevention; perinatal infection; pregnancy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Claeys, G State Univ Ghent, Dept Microbiol, Microbiol Lab, Pintelaan 185, B-9000 Ghent, Belgium State Univ Ghent Pintelaan 185 Ghent Belgium B-9000 nt, Belgium
Citazione:
G. Claeys et al., "Modified Granada Agar Medium for the detection of group B streptococcus carriage in pregnant women", CL MICRO IN, 7(1), 2001, pp. 22-24

Abstract

Objectives To improve the detection rate of group B streptococci (GBS) in pregnant women, aiming at the prevention of early-onset septicemia in the newborn. Methods The yield from culturing two sites, vaginal and anorectal, on a Modified Granada Medium (MGM) was compared with our standard approach of culturing a vaginal swab on blood agar (BA). Results Samples were processed from 430 consecutive pregnant women. GBS was isolated from the vagina in 11.6% with BA, and in 13.7% with MGM. In 17.0% of anorectal samples, GBS was identified with MGM. The combination of both sites and media had a yield of 20.0%. MGM identified all but six (2%) of 310 GBS strains after aerobic incubation, with use of a cover slide, and missed only three strains (1%) after anaerobic incubation. Conclusions Separate culture of vaginal and anorectal samples using the same MGM agar plate resulted in an increase in detection rate for GBS of 76% as compared to BA alone. The technique is simple and results are available after overnight incubation. MGM was confirmed as a specific medium for the identification of GBS, with a sensitivity of 98-99%.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 20:03:37