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Titolo:
Identification of Lens culinaris ssp culinaris chromosomes by physical mapping of repetitive DNA sequences
Autore:
Galasso, I; Schmidt, T; Pignone, D;
Indirizzi:
CNR, Ist Germoplasma, I-70126 Bari, Italy CNR Bari Italy I-70126CNR, Ist Germoplasma, I-70126 Bari, Italy Univ Kiel, Inst Crop Sci & Plant Breeding, D-24118 Kiel, Germany Univ Kiel Kiel Germany D-24118 i & Plant Breeding, D-24118 Kiel, Germany
Titolo Testata:
CHROMOSOME RESEARCH
fascicolo: 3, volume: 9, anno: 2001,
pagine: 199 - 209
SICI:
0967-3849(200104)9:3<199:IOLCSC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; RIBOSOMAL-RNA GENES; UNGUICULATA L WALP; GENUS LENS; AEGILOPS UMBELLULATA; SATELLITE DNA; RAPD ANALYSIS; VICIA-FABA; ORGANIZATION; WHEAT;
Keywords:
cytogenetic molecular markers; karyotype; Leguminoseae; repetitive DNA; Vicieae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Pignone, D CNR, Ist Germoplasma, Via G Amendola 165-A, I-70126 Bari, ItalyCNR Via G Amendola 165-A Bari Italy I-70126 -70126 Bari, Italy
Citazione:
I. Galasso et al., "Identification of Lens culinaris ssp culinaris chromosomes by physical mapping of repetitive DNA sequences", CHROMOS RES, 9(3), 2001, pp. 199-209

Abstract

We describe the characterisation and the chromosomal localisation of two repeated DNA sequences, named pLc30 (466 bp long, 64% AT residues) and pLc7 (408 bp long, 61% AT residues), isolated from lentil (Lens culinaris ssp. culinaris) genomic DNA. The pLc30 family is characterised by four internal repeats organised in a head-to-tail orientation, whereas the pLc7 contains many short direct subrepeats. The two families do not share significant sequence similarity. The distribution of these repetitive sequences in different Lens species and in other legumes was investigated. pLc30 is present in all Lens species investigated but absent from other genera examined. In contrast, pLc7 is present also in the genome of other legumes. As determined by FISH, the pLc30 sequence hybridises on six out of seven lentil chromosome pairs, while pLc7 hybridises on one only. The distributionof the nine different hybridisation sites of pLc30 allows the discrimination of all seven chromosome pairs and the construction of a karyotype of L. culinaris ssp. culinaris. Additionally, the combination of simultaneous andsuccessive FISH with pLc7, 5S rRNA, 18S-5.8S-25S rRNA genes, and a telomeric sequence allowed the assembly of a physical map based on lentil karyotype.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:39:34