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Titolo:
Regulation of the Hsp90-binding immunophilin, cyclophilin 40, is mediated by multiple sites for CA-binding protein (GABP)
Autore:
Kumar, P; Ward, BK; Minchin, RF; Ratajczak, T;
Indirizzi:
Sir Charles Gairdner Hosp, Dept Endocrinol & Diabet, Nedlands, WA 6009, Australia Sir Charles Gairdner Hosp Nedlands WA Australia 6009 , WA 6009, Australia Univ Western Australia, Dept Pharmacol, Queen Elizabeth II Med Ctr, Nedlands, WA 6009, Australia Univ Western Australia Nedlands WA Australia 6009 nds, WA 6009, Australia Western Australian Inst Med Res, Queen Elizabeth II Med Ctr, Nedlands, WA 6009, Australia Western Australian Inst Med Res Nedlands WA Australia 6009009, Australia
Titolo Testata:
CELL STRESS & CHAPERONES
fascicolo: 1, volume: 6, anno: 2001,
pagine: 78 - 91
SICI:
1355-8145(200101)6:1<78:ROTHIC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK-PROTEIN; PROGESTERONE-RECEPTOR COMPLEXES; GLUCOCORTICOID RECEPTOR; TRANSCRIPTION FACTOR; ESTROGEN-RECEPTOR; FK506-BINDING PROTEIN; MUTATIONAL ANALYSIS; MOLECULAR-CLONING; DNA METHYLATION; GENE-EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Ratajczak, T Sir Charles Gairdner Hosp, Dept Endocrinol & Diabet, Hosp Ave, Nedlands, WA 6009, Australia Sir Charles Gairdner Hosp Hosp Ave Nedlands WA Australia 6009
Citazione:
P. Kumar et al., "Regulation of the Hsp90-binding immunophilin, cyclophilin 40, is mediated by multiple sites for CA-binding protein (GABP)", CELL STR CH, 6(1), 2001, pp. 78-91

Abstract

Within steroid receptor heterocomplexes the large tetraticopeptide repeat-containing immunophilins, cyclophilin 40 (CyP40), FKBP51, and FKBP52, target a common interaction site in heat shock protein 90 (HspSO) and act coordinately with HspSO to modulate receptor activity. The reversible nature of the interaction between the immunophilins and HspSO suggests that relative cellular abundance might be a key determinant of the immunophilin component within steroid receptor complexes. To investigate CyP40 gene regulation, wehave isolated a fi-kilobase (kb) 5 ' -flanking region of the human gene and demonstrated that a similar to 50 base pair (bp) sequence adjacent to thetranscription start site is essential for CyP40 basal expression. Three tandemly arranged Ets sites within this critical region were identified as binding elements for the multimeric Ets-related transcription factor, GA binding protein (GABP). Functional studies of this proximal promoter sequence, in combination with mutational analysis, confirmed these sites to be crucial for basal promoter function. Furthermore, overexpression of both GABP alpha and GABP beta subunits in Cos1 cells resulted in increased endogenous CyP40 mRNA levels. Significantly, a parallel increase in FKBP52 mRNA expression was not observed, highlighting an important difference in the mode of regulation of the CyP40 and FKBP52 genes. Our results identify GABP as a key regulator of CyP40 expression. GAFF is a common target of mitogen and stress-activated pathways and may integrate these diverse extracellular signals to regulate CyP40 gene expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 08:57:46