Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Role of vascular endothelial growth factor in the stimulation of cellular invasion and signaling of breast cancer cells
Autore:
Price, DJ; Miralem, T; Jiang, SX; Steinberg, R; Avraham, H;
Indirizzi:
Harvard Univ, Beth Israel Deaconess Med Ctr, Div Expt Med, Inst Med, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 Expt Med, Inst Med, Boston, MA 02115 USA
Titolo Testata:
CELL GROWTH & DIFFERENTIATION
fascicolo: 3, volume: 12, anno: 2001,
pagine: 129 - 135
SICI:
1044-9523(200103)12:3<129:ROVEGF>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXON 7-ENCODED DOMAIN; FACTOR VEGF; TYROSINE KINASE; PHOSPHATIDYLINOSITOL 3-KINASE; RECEPTOR KDR; TUMOR-CELLS; EXPRESSION; ANGIOGENESIS; CARCINOMA; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Avraham, H Harvard Univ, Beth Israel Deaconess Med Ctr, Div Expt Med, InstMed, 4 Blackfan Circle, Boston, MA 02115 USA Harvard Univ 4 Blackfan Circle Boston MA USA 02115 MA 02115 USA
Citazione:
D.J. Price et al., "Role of vascular endothelial growth factor in the stimulation of cellular invasion and signaling of breast cancer cells", CELL GROWTH, 12(3), 2001, pp. 129-135

Abstract

The expression of vascular endothelial growth factor (VEGF) by breast tumors has been previously correlated with a poor prognosis in the pathogenesisof breast cancer. Furthermore, VEGF secretion is a prerequisite for tumor development. Although most of the effects of VEGF have been shown to be attributable to the stimulation of endothelial cells, we present evidence herethat breast tumor cells are capable of responding to VEGF. We show that VEGF stimulation of T-47D breast cancer cells leads to changes in cellular signaling and invasion. VEGF increases the cellular invasion of T-47D breast cancer cells on Matrigel/fibronectin-coated transwell membranes by a factorof two. Northern analysis for the expression of the known VEGF receptors shows the presence of moderate levels of Flt-1 and low levels of Flk-1/KDR mRNAs in a variety of breast cancer cell lines. T-47D breast cancer cells bind (125)l-labeled VEGF with a Kd of 13 x 10(-9) M. VEGF induces the activation of the extracellular regulated kinases 1,2 as well as activation of phosphatidylinositol 3'-kinase, Akt, and Forkhead receptor L1. These findings in T-47D breast cancer cells strongly suggest an autocrine role for VEGF contributing to the tumorigenic phenotype.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/06/20 alle ore 02:30:54