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Titolo:
Molecular detection of metastatic retinoblastoma cells by reverse transcription polymerase reaction for interphotoreceptor retinoid-binding protein mRNA - A case report
Autore:
Yamashita, N; Nishiuchi, R; Oda, M; Tomiyama, Y; Eguchi, N; Endo, C; Manki, A; Seino, Y;
Indirizzi:
Okayama Univ, Sch Med, Dept Pediat, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 , Dept Pediat, Okayama 7008558, Japan
Titolo Testata:
CANCER
fascicolo: 8, volume: 91, anno: 2001,
pagine: 1568 - 1573
SICI:
0008-543X(20010415)91:8<1568:MDOMRC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHAIN-REACTION; EXPRESSION; NEUROBLASTOMA; SEQUENCE; SUBUNIT; TUMORS; CDNA; GENE;
Keywords:
retinoblastoma; polymerase chain reaction; hematopoietic stem cell transplantation; retinol-binding proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Nishiuchi, R Okayama Univ, Sch Med, Dept Pediat, 2-5-1 Shikata Cho, Okayama 7008558, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008558 , Japan
Citazione:
N. Yamashita et al., "Molecular detection of metastatic retinoblastoma cells by reverse transcription polymerase reaction for interphotoreceptor retinoid-binding protein mRNA - A case report", CANCER, 91(8), 2001, pp. 1568-1573

Abstract

BACKGROUND. In the current study, the authors report a 4 year old girl with disseminated retinoblastoma. To find sensitive and specific molecular markers for detection of retinoblastoma cells in blood and marrow the authors evaluated three photoreceptor-associated gene transcripts by using reverse transcription polymerase chain reaction (RT-PCR). METHOD. Samples of bone marrow and blood were obtained from healthy donorsand the patient. RT-PCR was performed to detect the cone alpha'-subunit ofcGMP phosphodiesterase (cone alpha'-PDE), the rod beta -subunit of cGMP (rod beta -PDE), and the interphotoreceptor retinoid-binding protein (IRBP) gene transcript in RNA extracted from the samples. RESULTS, While no expression of rod beta -PDE or IRBP was detected in any of the normal samples, expression of cone alpha'-PDE was detected in two out of seven normal marrow samples. Expression of rod beta -PDE was not detected in the patient samples. Expression of IRBP was detected in the patient samples obtained from iliac bone marrow before intensive chemotherapy but not thereafter. CONCLUSION, RT-PCR for IRBP was a useful method for detecting metastatic retinoblastoma cells as well as for evaluating the therapeutic effects of treatment in this particular case. Cancer 2001;91:1568-73. (C) 2001 American Cancer Society.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:44:51