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Titolo:
Glucocorticoids and serotonin alter glucocorticoid receptor (GR) but not mineralocorticoid receptor (MR) mRNA levels in fetal mouse hippocampal neurons, in vitro
Autore:
Erdeljan, P; MacDonald, JF; Matthews, SG;
Indirizzi:
Univ Toronto, Fac Med, Dept Physiol, Toronto, ON M5S 1A8, Canada Univ Toronto Toronto ON Canada M5S 1A8 ysiol, Toronto, ON M5S 1A8, Canada Univ Toronto, Fac Med, Dept Obstet & Gynecol, Toronto, ON M5S 1A8, Canada Univ Toronto Toronto ON Canada M5S 1A8 necol, Toronto, ON M5S 1A8, Canada Univ Toronto, Fac Med, Dept Pharmacol, Toronto, ON M5S 1A8, Canada Univ Toronto Toronto ON Canada M5S 1A8 macol, Toronto, ON M5S 1A8, Canada
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 896, anno: 2001,
pagine: 130 - 136
SICI:
0006-8993(20010330)896:1-2<130:GASAGR>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PITUITARY-ADRENAL AXIS; GUINEA-PIG BRAIN; MESSENGER-RNA; ENVIRONMENTAL-REGULATION; DEXAMETHASONE TREATMENT; RAT; STRESS; HORMONES; BINDING; EXPRESSION;
Keywords:
glucocorticoids; serotonin; glucocorticoid receptors; mineralocorticoid receptors; hippocampus; development;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Matthews, SG Univ Toronto, Fac Med, Dept Physiol, Med Sci Bldg,1 Kings Circle, Toronto,ON M5S 1A8, Canada Univ Toronto Med Sci Bldg,1 Kings Circle Toronto ON Canada M5S 1A8
Citazione:
P. Erdeljan et al., "Glucocorticoids and serotonin alter glucocorticoid receptor (GR) but not mineralocorticoid receptor (MR) mRNA levels in fetal mouse hippocampal neurons, in vitro", BRAIN RES, 896(1-2), 2001, pp. 130-136

Abstract

Studies utilizing rats and guinea pigs have demonstrated that the hypothalamo-pituitary-adrenal (HPA) axis can be programmed by glucocorticoids during fetal life. Such programming is believed to occur, at least partially, atthe level of hippocampal glucocorticoid receptors (GR) and mineralocorticoid receptors (MR). Studies have also demonstrated that serotonin up regulates GR levels within the developing hippocampus. However, the cell type in which these changes take place has not been determined. We hypothesized thatdexamethasone, corticosterone and serotonin exposure modify GR and MR mRNAlevels in fetal mouse hippocampal cultures, and that these effects are confined to neurons. Cultures were derived from CD1 mouse fetuses on day IH ofgestation (n=8 dams). Fetal hippocampi were dissected, then mechanically and chemically dispersed. Cultures were exposed to dexamethasone. corticosterone or serotonin (1-100 nM) for 4 days. Levels of GR and MR mRNA were examined by in situ hybridization and high-resolution silver emulsion autoradiography. Four days exposure to dexamethasone or corticosterone (10 or 100 nM) decreased levels of CR mRNA within neurons. There was no significant change in MR mRNA in either experiment. Exposure to serotonin (100 nM) significantly increased expression of GR mRNA in hippocampal neurons. MR mRNA levels were unaffected by serotonin treatment. Dexamethasone, corticosterone or serotonin exposure did not alter expression of GR mRNA within glial cells. We conclude that synthetic and endogenous glucocorticoids, as well as serotonin, can influence neuronal levels of GR mRNA during hippocampal development. However, whether these effects are permanent remains to be determined. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 07/04/20 alle ore 22:10:24