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Titolo:
Interferon-gamma-induced changes in synaptic activity and AMPA receptor clustering in hippocampal cultures
Autore:
Vikman, KS; Owe-Larsson, B; Brask, J; Kristensson, KS; Hill, RH;
Indirizzi:
Karolinska Inst, Dept Neurosci, SE-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden SE-17177 ci, SE-17177 Stockholm, Sweden
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 896, anno: 2001,
pagine: 18 - 29
SICI:
0006-8993(20010330)896:1-2<18:ICISAA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; LONG-TERM POTENTIATION; DOMAIN-CONTAINING PROTEIN; NITRIC-OXIDE; MULTIPLE-SCLEROSIS; SILENT SYNAPSES; GLUTAMATE RECEPTORS; DENDRITIC SPINES; NMDA RECEPTOR; NEURONS;
Keywords:
nervous system; glutamate receptor; cytokine; viral infection; synapse; dendritic spine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Vikman, KS Karolinska Inst, Dept Neurosci, Nobels Vag 12A, SE-17177 Stockholm, Sweden Karolinska Inst Nobels Vag 12A Stockholm Sweden SE-17177 weden
Citazione:
K.S. Vikman et al., "Interferon-gamma-induced changes in synaptic activity and AMPA receptor clustering in hippocampal cultures", BRAIN RES, 896(1-2), 2001, pp. 18-29

Abstract

Extended release of interferon-gamma (IFN-gamma) in the nervous system during immunological and infectious conditions may trigger demyelinating disorders and cause disturbances in brain function. The aim of this study was toexamine the effects of IFN-gamma on neuronal function in rat hippocampal cell cultures by using whole cell patch clamp analysis together with quantitative immunocytochemistry. Acute application of IFN-gamma to differentiatedneurons in culture caused no immediate neurophysiological responses, hut recordings after 48 h of incubation displayed an increase in frequency of AMPA receptor (AMPAR)-mediated spontaneous excitatory postsynaptic currents (EPSCs). Quantitative immunocytochemistry for the AMPAR subunit GluR1 showedno alteration in receptor clustering at this time point. However, prolonged treatment with IFN-gamma for 2 weeks resulted in a significant reduction in AMPAR clustering on dendrites but no marked differences in EPSC frequency between treated neurons and controls could be observed. On the other hand, treatment of hippocampal neurons for 4 weeks, instituted at an immature stage (1 day in culture), caused a significant reduction in spontaneous EPSCfrequency. These neurons developed with no overt alterations in dendritic arborization or in the appearance of dendritic spines as visualized by alpha -actinin immunocytochemistry. Nonetheless, there was a marked reduction in AMPAR clustering on dendrites. These observations show that a key immunomodulatory molecule, IFN-gamma, can cause long-term modifications of synaptic activity and perturb glutamate receptor clustering. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 10:22:10