Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Latex particles with thermo-flocculation and magnetic properties for immobilization of alpha-chymotrypsin
Autore:
Chen, JP; Su, DR;
Indirizzi:
Chang Gung Univ, Dept Chem & Mat Engn, Taoyuan 333, Taiwan Chang Gung Univ Taoyuan Taiwan 333 Chem & Mat Engn, Taoyuan 333, Taiwan
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 2, volume: 17, anno: 2001,
pagine: 369 - 375
SICI:
8756-7938(200103/04)17:2<369:LPWTAM>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
INVERSE MICROEMULSION POLYMERIZATION; ENZYME IMMOBILIZATION; HYDROGEL MICROSPHERES; BEADS; METHACRYLATE; ADSORPTION; STABILITY; SUPPORTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, JP Chang Gung Univ, Dept Chem & Mat Engn, Taoyuan 333, Taiwan Chang Gung Univ Taoyuan Taiwan 333 at Engn, Taoyuan 333, Taiwan
Citazione:
J.P. Chen e D.R. Su, "Latex particles with thermo-flocculation and magnetic properties for immobilization of alpha-chymotrypsin", BIOTECH PR, 17(2), 2001, pp. 369-375

Abstract

Core-shell-type latex particles composed of styrene, N-isopropylacrylamide(NIPAAm), and N-acryloxysuccinimide (NAS) were synthesized by surfactant-free emulsion polymerization. The latex particles show thermo-flocculation behavior due to the presence of temperature-sensitive monomer NIPAAm and could be used for immobilization of cr-chymotrypsin through covalent bonding with the reactive ester groups of NAS. Enzyme recycle could be accomplished in this immobilized enzyme system by sedimentation of the thermo-flocculated latex particles in 20 min at 30 degreesC by raising the salt (NaCl) concentration to 0.5 M. To further enhance the sedimentation rate, ultrafine magnetite particles were prepared and included during polymerization to produce magnetic temperature-sensitive latex particles (MTLP), which could be recovered 6 times faster after thermo-flocculation by applying a low magnetic field. However; a higher salt concentration was necessary to flocculate theMTLP under the same condition as a result of its increased surface hydrophilicity, which originates from different polymerization conditions and the incorporation of magnetite. The immobilized enzyme shows high activity evenagainst macromolecular substrates (hemoglobin and casein) owing to limiteddiffusion resistance, with full activity retention for nonmagnetic latex but one-half reduction in activity if the magnetic property was introduced. Optimal enzyme immobilization pH and enzyme loading were determined, and properties of the immobilized enzyme were characterized. The immobilized enzyme was used in 10 repeated batch hydrolyses of casein with successive flocculation/dispersion cycles and showed less than 15% activity decrease at theend. Overall, introducing the magnetic property to the latex could effectively enhance the solid-liquid separation rate after thermo-flocculation andmaintain enzyme activity after repeated use but adversely influence the activity of the immobilized enzyme.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:10:43