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Titolo:
Impact of cell growth morphology on retroviral transduction: Effect of contact inhibition
Autore:
Kwon, YJ; Peng, CA;
Indirizzi:
Univ So Calif, Dept Chem Engn, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 em Engn, Los Angeles, CA 90089 USA
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 2, volume: 17, anno: 2001,
pagine: 240 - 246
SICI:
8756-7938(200103/04)17:2<240:IOCGMO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-TRANSFER; VECTORS; INFECTION; THERAPY; EXPRESSION; TIME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Peng, CA Univ So Calif, Dept Chem Engn, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 Los Angeles, CA 90089 USA
Citazione:
Y.J. Kwon e C.A. Peng, "Impact of cell growth morphology on retroviral transduction: Effect of contact inhibition", BIOTECH PR, 17(2), 2001, pp. 240-246

Abstract

Retrovirus-mediated gene transfer is one of the most commonly used methodsto deliver, integrate, and express the gene of interest because the retrovirus can insert the desired gene into the chromosome of the target cells with high stability. However, to deliver the gene successfully, the retrovirus requires active division to integrate reversely transcribed DNA into the chromosome of target cells. In this study, we focused on the effect of cell-cell contact inhibition on the efficiency of retroviral transduction with two anchorage-dependent cell lines: NIH 3T3 and 293 cells. These two cell lines have very different cell morphologies and growth patterns on surfaces. Human embryonic kidney epithelial 293 cells tend to stick together after dividing, while NIH 3T3 cells migrate to occupy available surface and spread. Experimental data indicate that the abatement of the transduction rate of293 cells was initiated in the early stage of the culture, whereas effect of contact inhibition of NIH 3T3 cells on the transduction rate became dominating at the end of the culture period. Experimental results were also quantitatively illustrated by plotting normalized multiplicity of infection (MOI) versus normalized cell density. According to the outcomes, cell inoculation density plays an important role in optimizing the retroviral transduction rate. The optimal time of retroviral transduction should be confined tothe accelerating growth phase for 293 cells and at the exponential growth phase for NIH 3T3 cells. The implication drawn from this study is that contact inhibition effect on retroviral transduction should be taken into account for large-scale gene transfer systems such as the microcarrier bioreactor.

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Documento generato il 20/02/20 alle ore 03:01:06