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Titolo:
Experimental regulation of STAT gene expression reveals an involvement of STAT5 in interleukin-4-driven cell proliferation
Autore:
Friedrich, K; Wietek, S;
Indirizzi:
Theodor Boveri Inst Biowissensch, Bioctr, Dept Physiol Chem 2, D-97074 Wurzburg, Germany Theodor Boveri Inst Biowissensch Wurzburg Germany D-97074 zburg, Germany
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 2, volume: 382, anno: 2001,
pagine: 343 - 351
SICI:
1431-6730(200102)382:2<343:EROSGE>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; RECEPTOR-BETA CHAIN; MAMMALIAN-CELLS; TYROSINE RESIDUES; GAMMA-CHAIN; ACTIVATION; IL-4; RESPONSES; PROTEINS; PROMOTER;
Keywords:
cell proliferation; cytokine receptors; JAK; STAT pathway; regulatable gene expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Friedrich, K Univ Jena, Inst Biochem, Nonnenplan 2, D-07743 Jena, Germany Univ Jena Nonnenplan 2 Jena Germany D-07743 43 Jena, Germany
Citazione:
K. Friedrich e S. Wietek, "Experimental regulation of STAT gene expression reveals an involvement of STAT5 in interleukin-4-driven cell proliferation", BIOL CHEM, 382(2), 2001, pp. 343-351

Abstract

The precise roles of signal transducers and activators of transcription (STATs) in cytokine-triggered control of cell physiology are not sufficientlywell understood. We have established cell lines in which the individual functional contributions of STAT6 and STAT5a/b to interleukin-(IL-) 3 and -4-dependent processes can be readily studied. Mutants of STAT6, STAT5a and 5blacking the transcriptional transactivation domain were fused to the greenfluorescent protein (GFP) and expressed in the murine pro-B cell line Ba/F3 in a regulatable fashion. The expression of these truncated STAT variantscould be tightly controlled over a wide range by doxycycline in the medium. They specifically bound to cognate DNA elements upon cytokine stimulationand acted dominant-negatively on the transcription of respective reporter genes in response to IL-3 and -4. The system was applied to the question ofSTAT contributions to cytokine-dependent cell proliferation. Expression ofdominant-negative STAT6 had no significant effect on cell growth in response to both IL-3 and IL-4. In contrast, truncated STATE interfered with cellproliferation in response to IL-3, and, interestingly, also to IL-4. The results support our earlier findings on a role of STAT5 in IL-4-induced intracellular signaling and indicate that STAT5b in particular is involved in IL-4 receptor-triggered control of cell proliferation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:52:49