Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Ethyl docosahexaenoate-associated decrease in fetal brain lipid peroxide production is mediated by activation of prostanoid and nitric oxide pathways
Autore:
Green, P; Glozman, S; Yavin, E;
Indirizzi:
Weizmann Inst Sci, Dept Neurobiol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 biol, IL-76100 Rehovot, Israel
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 1-2, volume: 1531, anno: 2001,
pagine: 156 - 164
SICI:
1388-1981(20010330)1531:1-2<156:EDDIFB>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYUNSATURATED FATTY-ACIDS; CYCLOOXYGENASE-2 GENE-EXPRESSION; RAT-BRAIN; CELLS; PROSTAGLANDIN; CONSTITUENT; SUPEROXIDE; GENERATION; SYNTHASE; ISCHEMIA;
Keywords:
arachidonic acid metabolism; phospholipid; cyclooxygenase; phospholipase A(2); docosahexaenoic acid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Yavin, E Weizmann Inst Sci, Dept Neurobiol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 76100 Rehovot, Israel
Citazione:
P. Green et al., "Ethyl docosahexaenoate-associated decrease in fetal brain lipid peroxide production is mediated by activation of prostanoid and nitric oxide pathways", BBA-MOL C B, 1531(1-2), 2001, pp. 156-164

Abstract

Previously we have shown that intraamniotic administration of ethyl docosahexaenoate (Et-DHA) to pregnant rats resulted in decreased lipid peroxidation in the fetal brain, under a variety of conditions (S. Glozman, P. Green,E. Yavin, J. Neurochem. 70 (1998) 2482-2491). In the present study we examine the potential mechanisms to explain this effect. This was done by a pharmacological approach, utilizing brain slice preparations from Et-DHA treated or control rats in the presence of various agents and examining the formation of products in the tissue slices or incubation medium. Et-DHA treatedbrains produced 2-3-foId more prostanoids (PN) than control brains, indicating cyclooxygenase (COX) activation. Indomethacin at 50 muM inhibited PN formation and also abolished Et-DHA induced decrease in lipid peroxides, as evident by the levels of thiobarbituric acid reactive substances (TBARS) released in the medium. The phospholipase A(2) inhibitors quinacrine and p-bromophenacyl bromide added at 0.1 mM concentration each to either slices from controls or Et-DHA treated fetal brains, decreased TBARS production. Et-DHA treated brains released 2.2-fold more nitric oxide (NO) than control brains and NO synthase (NOS) inhibitors abolished this effect. Increasing the concentration of NO by the addition of an NO donor greatly decreased the concentration of the TEARS in the medium. These results suggest that at leastsome of the effect of Et-DKA on decreased lipid peroxidation may be explained by a shift of oxygen species utilization via enzymaticatlly regulated, therefore metabolically controlled, COX and NOS activities. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 09:51:43