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Titolo:
The relationship between H-1-NMR mobile lipid intensity and cholesterol intwo human tumor multidrug resistant cell lines (MCF-7 and LoVo)
Autore:
Santini, MT; Romano, R; Rainaldi, G; Filippini, P; Bravo, E; Porcu, L; Motta, A; Calcabrini, A; Meschini, S; Indovina, PL; Arancia, G;
Indirizzi:
Ist Super Sanita, Ultrastrutture Lab, I-00161 Rome, Italy Ist Super Sanita Rome Italy I-00161 rastrutture Lab, I-00161 Rome, Italy Ist Nazl Fis Mat, Unita Napoli, I-80126 Naples, Italy Ist Nazl Fis Mat Naples Italy I-80126 nita Napoli, I-80126 Naples, Italy Univ Naples Federico II, Dipartimento Sci Fis, I-80126 Naples, Italy Univ Naples Federico II Naples Italy I-80126 Fis, I-80126 Naples, Italy Ist Chim Mol Interesse Biol, Ist Chim SIstemi Biol CHEMBIOS, I-00161 Rome,Italy Ist Chim Mol Interesse Biol Rome Italy I-00161 MBIOS, I-00161 Rome,Italy CNR, I-80125 Naples, Italy CNR Naples Italy I-80125CNR, I-80125 Naples, Italy
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 1-2, volume: 1531, anno: 2001,
pagine: 111 - 131
SICI:
1388-1981(20010330)1531:1-2<111:TRBHML>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAGNETIC-RESONANCE SPECTROSCOPY; PLASMA-MEMBRANE CHOLESTEROL; P-GLYCOPROTEIN; CANCER-CELLS; TRANSFORMED-CELLS; HUMAN NEUTROPHILS; NMR-SPECTROSCOPY; WHOLE CELLS; RAT-BRAIN; NILE RED;
Keywords:
multidrug resistance; LH-NMR; mobile lipid; cholesterol; P-glycoprotein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Santini, MT Ist Super Sanita, Ultrastrutture Lab, Viale Regina Elena 299, I-00161 Rome, Italy Ist Super Sanita Viale Regina Elena 299 Rome Italy I-00161 ly
Citazione:
M.T. Santini et al., "The relationship between H-1-NMR mobile lipid intensity and cholesterol intwo human tumor multidrug resistant cell lines (MCF-7 and LoVo)", BBA-MOL C B, 1531(1-2), 2001, pp. 111-131

Abstract

The high resolution proton nuclear magnetic resonance (H-1-NMR) spectra oftwo different cell lines exhibiting multidrug resistance (MDR) as demonstrated by the expression of the well-known energy-driven, membrane-bound 170 kDa P-glycoprotein pump known as Pgp were investigated. In particular, the mobile lipid (ML) profile, and the growth and biochemical characteristics of MCF-7 (human mammary carcinoma) and LoVo (human colon adenocarcinoma) sensitive and resistant tumor cells were compared. The results indicate that both MCF-7 and LoVo resistant cells have a higher ML intensity than their respective sensitive counterparts. However, since sensitive and resistant cells of each pair grow in the same manner, variations in growth characteristics do not appear to be the cause of the ML changes as has been suggested byother authors in non-resistant tumor cells. In order to investigate further the origin of the ML changes, lipid analyses were conducted in sensitive and resistant cell types. The results of these experiments show that resistant cells of both cell types have a greater amount of esterified cholesterol and saturated cholesteryl ester and triglyceride fatty acid than their sensitive counterparts. From a thorough analysis of the data obtained in thispaper utilizing numerous techniques including biological, biophysical and biochemical ones, it is hypothesized that cholesterol and triglyceride playa pivotal role in inducing changes in NMR ML signals. The importance of these lipid variations in MDR is discussed in view of the controversy regarding the origin of ML signals and the paramount role played by the Pgp pump iq resistance. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 28/03/20 alle ore 23:18:44