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Titolo:
Impaired spreading of surfactant phospholipids in the lungs of newborn rats with pulmonary hypoplasia as a model of congenital diaphragmatic hernia induced by nitrofen
Autore:
Utsuki, T; Hashizume, K; Iwamori, M;
Indirizzi:
Kinki Univ, Fac Sci & Technol, Dept Biochem, Higashiosaka, Osaka 5778502, Japan Kinki Univ Higashiosaka Osaka Japan 5778502 hiosaka, Osaka 5778502, Japan Kiyosenomori Hosp, Dept Pediat, Kiyose, Tokyo 2040021, Japan Kiyosenomori Hosp Kiyose Tokyo Japan 2040021 Kiyose, Tokyo 2040021, Japan Univ Tokyo, Grad Sch Med, Dept Pediat Surg, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 iat Surg, Bunkyo Ku, Tokyo 1130033, Japan
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 1-2, volume: 1531, anno: 2001,
pagine: 90 - 98
SICI:
1388-1981(20010330)1531:1-2<90:ISOSPI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY-DISTRESS SYNDROME; II CELLS; DISATURATED PHOSPHATIDYLCHOLINE; EPITHELIAL-CELLS; SENSITIVE METHOD; SECRETION; ULTRASTRUCTURE; STIMULATION; MATURATION;
Keywords:
amniotic fluid; congenital diaphragmatic hernia; disaturated phosphatidylcholine; pulmonary alveoli; sphingomyelin; thin layer chromatography-immunostaining;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Iwamori, M Kinki Univ, Fac Sci & Technol, Dept Biochem, 3-4-1 Kowakae, Higashiosaka, Osaka 5778502, Japan Kinki Univ 3-4-1 Kowakae Higashiosaka OsakaJapan 5778502 Japan
Citazione:
T. Utsuki et al., "Impaired spreading of surfactant phospholipids in the lungs of newborn rats with pulmonary hypoplasia as a model of congenital diaphragmatic hernia induced by nitrofen", BBA-MOL C B, 1531(1-2), 2001, pp. 90-98

Abstract

In order to clarify the pathological outcome of congenital diaphragmatic hernia (CDH), we devised an animal model of CDH by administration of 2,4-dichlorophenyl-p-nitrophenyl ether (nitrofen) to pregnant rats, and determinedthe level and distribution of lung surfactant using the monoclonal antibody toward sphingomyelin and disaturated phosphatidylcholine (disat-PC), In control rats, the concentration of disat-PC was found to increase greatly from 16 to 18 days of gestation. Intragastric administration of nitrofen to pregnant rats at day 9 of gestation resulted in CDH in 42.7% of fetuses delivered after 20 days of gestation. In nitrofen-treated fetuses, the concentration of disat-PC in the lungs was lower than those in control fetuses, andsurfactant apoprotein SP-A was similarly reduced in nitrofen-treated fetuses. However, the concentration of disat-PC in nitrofen-treated fetuses was higher than that in control fetuses at 18 days of gestation, indicating a synthetic potential of surfactant in nitrofen-treated fetuses comparable to that at the late stage of normal gestation. Immunohistochemical study with the antibody revealed that surfactant phospholipid was mainly in the form of intracellular granules in nitrofen-treated fetuses, probably causing the hypoplastic lungs and then CDH, in contrast to the uniform distribution on the pulmonary alveolar surface in control fetuses. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:13:36