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Titolo:
Immunohistochemical localization of the neural cell adhesion molecule in the rat sciatic nerve
Autore:
Dolapchieva, S; Eggers, R; Kuhnel, W;
Indirizzi:
Med Univ Sofia, Dept Anat & Histol, BR-1431 Sofia, Bulgaria Med Univ Sofia Sofia Bulgaria BR-1431 & Histol, BR-1431 Sofia, Bulgaria Med Univ Lubeck, Inst Anat, D-23538 Lubeck, Germany Med Univ Lubeck Lubeck Germany D-23538 nst Anat, D-23538 Lubeck, Germany
Titolo Testata:
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
fascicolo: 2, volume: 183, anno: 2001,
pagine: 129 - 133
SICI:
0940-9602(200103)183:2<129:ILOTNC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYELIN-ASSOCIATED GLYCOPROTEIN; N-CAM; RESTRICTED LOCALIZATION; CARBOHYDRATE EPITOPE; EXPRESSION; L1; NCAM; CULTURE; ROLES; INNERVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Dolapchieva, S Med Univ Sofia, Dept Anat & Histol, Zdrave Str 2, BR-1431 Sofia, Bulgaria Med Univ Sofia Zdrave Str 2 Sofia Bulgaria BR-1431 ulgaria
Citazione:
S. Dolapchieva et al., "Immunohistochemical localization of the neural cell adhesion molecule in the rat sciatic nerve", ANN ANATOMY, 183(2), 2001, pp. 129-133

Abstract

The neural cell adhesion molecule (NCAM) has been widely studied in the early embryonal. development of the nervous system. The data about NCAM distribution in the peripheral nerves during postnatal life are scant and some controversial. In the present study, the NCAM localization in the sciatic nerves of 15-day-old Wistar rats has been studied. Semi-thin sections of the nerves were immunotested with a polyclonal antibody (Santa Cruz Biotechnology) that recognizes rat NCAM. The antibody was visualized with donkey anti-goat IgG, conjugated to 12 nm colloidal gold, and silver amplification. In the myelinated nerve fibres, the immunoreactivity was associated with the axons, mainly with their plasma membrane, which was unstained in the nodes of Ranvier. The myelin sheaths and the myelinating Schwann cells were negative. The extracellular matrix and the bundles of non-myelinated nerve fibreswere immunopositive.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:20:32