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Titolo:
Rapid detection of Mycobacterium tuberculosis infection by enumeration of antigen-specific T cells
Autore:
Lalvani, A; Pathan, AA; McShane, H; Wilkinson, RJ; Latif, M; Conlon, CP; Pasvol, G; Hill, AVS;
Indirizzi:
Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Med, Oxford OX3 9DU, England Univ Oxford Oxford England OX3 9DU ept Clin Med, Oxford OX3 9DU, England Univ London Imperial Coll Sci Technol & Med, Northwick Pk Hosp, Wellcome Ctr Clin Trop Med, London, England Univ London Imperial Coll Sci Technol & Med London England don, England
Titolo Testata:
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
fascicolo: 4, volume: 163, anno: 2001,
pagine: 824 - 828
SICI:
1073-449X(200103)163:4<824:RDOMTI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESAT-6 ANTIGEN; PROTECTIVE IMMUNITY; BOVIS BCG; RESPONSES; SENSITIVITY; VACCINES; PROTEIN; RECALL; KENYA; MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Lalvani, A Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Med, Level7, Oxford OX3 9DU, England Univ Oxford Level 7 Oxford England OX3 9DU rd OX3 9DU, England
Citazione:
A. Lalvani et al., "Rapid detection of Mycobacterium tuberculosis infection by enumeration of antigen-specific T cells", AM J R CRIT, 163(4), 2001, pp. 824-828

Abstract

There is no reliable means of detecting latent M. tuberculosis infection, and even in patients with active tuberculosis, infection is often unconfirmed. We hypothesized that M. tuberculosis antigen-specific T cells might reliably indicate infection. We enumerated peripheral blood-derived interferongamma (IFN-gamma)-secreting T cells responding to epitopes from ESAT-6, anantigen that is highly specific for M. tuberculosis complex but absent from BCC, in four groups of individuals. Forty-five of 47 patients with bacteriologically confirmed tuberculosis had ESAT-6-specific IFN-gamma -secretingT cells, compared with four of 47 patients with nontuberculous illnesses, indicating that these T cells are an accurate marker of M. tuberculosis infection. This assay thus has a sensitivity of 96% (95% confidence interval [CI] 92-100) for detecting M. tuberculosis infection in this patient population. By comparison, of the 26 patients with tuberculosis who had a diagnostic: tuberculin skin test (TST), only 18 (69%) were positive (p = 0.003). Inaddition, 22 of 26 (85%) TST-positive exposed household contacts had ESAT-6-specific T cells, whereas zero of 26 unexposed BCC-vaccinated subjects responded. This approach enables rapid detection of M. tuberculosis infectionin patients with active tuberculosis and in exposed asymptomatic individuals at high risk of latent infection; it also successfully distinguishes between M, tuberculosis infection and BCC vaccination. This capability may facilitate tuberculosis control in nonendemic regions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 01:42:06