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Titolo:
Interleukin-6 up-regulates the oxytocin receptor in cultured uterine smooth muscle cells
Autore:
Rauk, PN; Friebe-Hoffmann, U; Winebrenner, LD; Chiao, JP;
Indirizzi:
Magee Womens Res Inst, Pittsburgh, PA 15213 USA Magee Womens Res Inst Pittsburgh PA USA 15213 t, Pittsburgh, PA 15213 USA Carlow Coll, Pittsburgh, PA 15213 USA Carlow Coll Pittsburgh PA USA 15213Carlow Coll, Pittsburgh, PA 15213 USA Univ Pittsburgh, Dept Obstet Gynecol & Reprod Sci, Div Maternal Fetal Med,Pittsburgh, PA USA Univ Pittsburgh Pittsburgh PA USA Maternal Fetal Med,Pittsburgh, PA USA
Titolo Testata:
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
fascicolo: 3, volume: 45, anno: 2001,
pagine: 148 - 153
SICI:
1046-7408(200103)45:3<148:IUTORI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRETERM LABOR; INFLAMMATORY CYTOKINES; PREMATURE LABOR; NUCLEAR FACTOR; EXPRESSION; GENE; PREGNANCY; TERM; BIOSYNTHESIS; PARTURITION;
Keywords:
human uterus; interleukin-6; oxytocin receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Rauk, PN Magee Womens Res Inst, 204 Craft Ave, Pittsburgh, PA 15213 USA Magee Womens Res Inst 204 Craft Ave Pittsburgh PA USA 15213 3 USA
Citazione:
P.N. Rauk et al., "Interleukin-6 up-regulates the oxytocin receptor in cultured uterine smooth muscle cells", AM J REPROD, 45(3), 2001, pp. 148-153

Abstract

PROBLEM: Intra-uterine infection results in the production of inflammatorycytokines, including interleukin-6 (IL-6). Increased oxytocin-receptor (OTR) concentrations are associated with the onset of preterm labor. We hypothesize that infection up-regulates OTR expression through IL-6-induced transcription factors. METHOD OF STUDY: Primary cultures of human myometrium were treated for various time periods or with different concentrations of IL-6 and OTR mRNA as well as OTR binding were measured by means of reverse transcription polymerase chain reaction and I-125-ornithine-vasotocin-binding assay. To study underlying mechanisms of OTR changes with IL-6 treated, cells were also incubated with genistein or H7 (tyrosine and serine phosphorylation inhibitors),respectively. RESULTS: OTR mRNA increased 2.5-fold after 4 hr of IL-6 treatment and OTR binding 1.4-fold after 8 hr of cytokine stimulation. The IL-6-induced increase in binding was blocked by genistein and H7. CONCLUSIONS: IL-6 up-regulates uterine OTR mRNA expression and binding capacity in cultured human myocytes most likely through tyrosine and serine phosphorylation pathways involving the nuclear factor STAT-3.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:39:40