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Titolo:
Correlation of hematopoietic progenitor cell count determined by the SE-9000 (TM) automated hematology analyzer with CD34(+) cell count by flow cytometry in leukapheresis products
Autore:
Park, KU; Kim, SH; Suh, C; Kim, S; Lee, SJ; Park, JS; Cho, HJ; Kim, KW; Lee, K; Kim, HJ; Park, J; Min, YJ; Kim, JG; Kim, T; Lee, JH; Kim, SB; Kim, SW; Lee, KH; Lee, JS; Kim, WK; Park, CJ; Chi, HS;
Indirizzi:
Dongguk Univ, Coll Med, Dept Med, Kyongju 780350, Kyongbuk, South Korea Dongguk Univ Kyongju Kyongbuk South Korea 780350 0, Kyongbuk, South Korea Univ Ulsan, Coll Med, Asan Med Ctr, Dept Med, Seoul, South Korea Univ Ulsan Seoul South Korea Asan Med Ctr, Dept Med, Seoul, South Korea Univ Ulsan, Coll Med, Asan Med Ctr, Dept Clin Pathol, Seoul, South Korea Univ Ulsan Seoul South Korea Ctr, Dept Clin Pathol, Seoul, South Korea
Titolo Testata:
AMERICAN JOURNAL OF HEMATOLOGY
fascicolo: 1, volume: 67, anno: 2001,
pagine: 42 - 47
SICI:
0361-8609(200105)67:1<42:COHPCC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; NON-HODGKINS-LYMPHOMA; BLOOD STEM-CELLS; PERIPHERAL-BLOOD; DOSE CYCLOPHOSPHAMIDE; TRANSPLANTATION; MOBILIZATION; CHEMOTHERAPY; ENGRAFTMENT; COLLECTIONS;
Keywords:
peripheral blood stem cell; flow cytometry; CD34(+) cells; automated hematology analyzer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Park, KU Dongguk Univ, Coll Med, Dept Med, 1090-1 Sukjang Dong, Kyongju 780350, Kyongbuk, South Korea Dongguk Univ 1090-1 Sukjang Dong Kyongju Kyongbuk South Korea 780350
Citazione:
K.U. Park et al., "Correlation of hematopoietic progenitor cell count determined by the SE-9000 (TM) automated hematology analyzer with CD34(+) cell count by flow cytometry in leukapheresis products", AM J HEMAT, 67(1), 2001, pp. 42-47

Abstract

The yield of stem cell collection after mobilization is crucial for autologous peripheral blood stem cell (PBSC) transplantation. Quantitative determinations of CD34(+) cells using flow cytometry or stem cell culture have been used, but these methods require much time, technical experience, and expensive reagents. The automated hematology analyzer (Sysmex SE-9000(TM), TQA, Japan) equipped with the Immature information (IMI) channel for immature myeloid cells can detect IMI+ cells within 90 sec. Detection is made possible by the combination of a special reagent system and direct current/radiofrequency biosensors. We studied the relation of IMI+ cells and variable cell counts with CD34(+) cell yield in autologous stem cell harvest. In a series of 32 patients (median age, 44 years: M:F = 11:21), 184 leukaphereses were performed after mobilization regimens with chemotherapy and G-CSF or G-CSF alone. Full blood cell counts were enumerated on peripheral blood (PB) samples taken prior to each leukapheresis. Mononuclear cell (MNC) and IMI+ cell counts by automated hematology analyzer and flow cytometry based CD34(+) cell yield were measured on the harvested product. The relationship amongPB white blood cells (WBC), PB monocytes, IMI+ cells, MNC, and CD34(+) cell yield in a single leukapheresis was estimated by Pearson correlation analysis. PB WBC count showed no correlation with CD34(+) cell yield in a single leukapheresis (r = 0.02, P = 0.81). PB monocyte count showed a weak correlation (r = 0.21, P = 0.01) and MNC in harvest also showed a weak correlation (r = 0.36, P = 0.0001) with CD34(+) cell yield. In contrast, CD34(+) cell yield correlated well with IMI+ cell count (r = 0.68, P = 0.0001), and data could be fitted by a linear regression equation, y = 0.330 + 0.974x. IMI cell assay by the automated hematology analyzer correlated well with the CD34(+) cell yield in a mobilized autologous stem cell harvest. The IMI+ cell count might be used as a simple and efficient indicator of blood stem cell mobilization and collection. Am. J. Hematol. 67:42-47, 2001. (C) 2001 Wiley-Liss. Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 21:03:31