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Titolo:
PREDICTION OF POLYPHENOL OXIDASE ACTIVITY IN MODEL SOLUTIONS CONTAINING VARIOUS COMBINATIONS OF CHLOROGENIC ACID, (-)-EPICATECHIN, O-2, CO2, TEMPERATURE, AND PH BY MULTIWAY DATA-ANALYSIS
Autore:
HEIMDAL H; BRO R; LARSEN LM; POLL L;
Indirizzi:
ROYAL VET & AGR UNIV,LMC CTR ADV FOOD STUDIES,DEPT CHEM,ROLIGHEDSVEJ 30 DK-1958 FREDERIKSBERG C DENMARK ROYAL VET & AGR UNIV,LMC CTR ADV FOOD STUDIES,DEPT CHEM DK-1958 FREDERIKSBERG C DENMARK ROYAL VET & AGR UNIV,LMC CTR ADV FOOD STUDIES,DEPT DAIRY & FOOD SCI DK-1958 FREDERIKSBERG C DENMARK
Titolo Testata:
Journal of agricultural and food chemistry
fascicolo: 7, volume: 45, anno: 1997,
pagine: 2399 - 2406
SICI:
0021-8561(1997)45:7<2399:POPOAI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANS-CAFFEOYLTARTARIC ACID; CAFFEIC ACID; ENZYMATIC OXIDATION; PHENOLIC-COMPOUNDS; LACTUCA-SATIVA; APPLE; SYSTEM; PURIFICATION; MECHANISM; INHIBITOR;
Keywords:
LETTUCE; LACTUCA SATIVA; PPO; ENZYMATIC BROWNING ATMOSPHERE; PACKAGING; CA; MA; HPLC; POLAROGRAPHIC ASSAY; UV-VIS SPECTRA; MULTIWAY DATA ANALYSIS; PLS; PARAFAC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
H. Heimdal et al., "PREDICTION OF POLYPHENOL OXIDASE ACTIVITY IN MODEL SOLUTIONS CONTAINING VARIOUS COMBINATIONS OF CHLOROGENIC ACID, (-)-EPICATECHIN, O-2, CO2, TEMPERATURE, AND PH BY MULTIWAY DATA-ANALYSIS", Journal of agricultural and food chemistry, 45(7), 1997, pp. 2399-2406

Abstract

Controlled or modified packaging (CA/MA) of lettuce is simulated in model solutions containing lettuce polyphenol oxidase (PPO), chlorogenic acid (CG), and/or (-)-epicatechin (EPI) under various combinations of CO2, O-2, N-2, temperature, and pH conditions. Enzymatic browning ismeasured by PPO activity, color formation, substrate consumption, anddevelopment of products. Results were analyzed by multiway data analysis. CO2 minimized the chemical polymerization and reduced PPO activity slightly. CG was a better substrate for PPO than EPI. CG was, in particular, involved in the primary enzymatic catalyzed oxidation, whereas EPI was important for the subsequent chemical polymerization in mixtures of CG and EPI. A very descriptive five-parameter multiplicative model was obtained from the PPO data by the multivariate method PARAFAC. This model describes the importance of substrate, atmospheric composition, pH, and temperature for PPO activity. Low pH (3.0) reduced PPO activity strongly (89%), whereas low temperature (5 degrees C) had a weaker effect on PPO activity (40% reduction of activity). The prediction of PPO activity (O-2 consumption) by kinetic UV-vis spectra correlated well (r(2) = 0.87) with traditional polarographic PPO assay.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:55:27