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Titolo:
Detection of specific IgE-antibodies (sIgE-AB) against natural latex (NL) in serum by means of the immunoblot and CAST in cases of misdetection in the CAP-FEIA-system or in the Uni CAP 100-system and in the AlaSTAT-system
Autore:
Hipler, UC; Bauer, A; Elsner, P;
Indirizzi:
Univ Jena, Klin Hautkrankheiten, D-07740 Jena, Germany Univ Jena Jena Germany D-07740 in Hautkrankheiten, D-07740 Jena, Germany
Titolo Testata:
ALLERGOLOGIE
fascicolo: 3, volume: 24, anno: 2001,
pagine: 100 - 106
SICI:
0344-5062(200103)24:3<100:DOSI(A>2.0.ZU;2-3
Fonte:
ISI
Lingua:
GER
Soggetto:
SKIN-PRICK TEST; SPINA-BIFIDA; ALLERGY; DIAGNOSIS;
Keywords:
natural latex; allergy; immunoblot;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Hipler, UC Univ Jena, Klin Hautkrankheiten, Erfurter Str 35, D-07740 Jena,Germany Univ Jena Erfurter Str 35 Jena Germany D-07740 0 Jena, Germany
Citazione:
U.C. Hipler et al., "Detection of specific IgE-antibodies (sIgE-AB) against natural latex (NL) in serum by means of the immunoblot and CAST in cases of misdetection in the CAP-FEIA-system or in the Uni CAP 100-system and in the AlaSTAT-system", ALLERGOLOGI, 24(3), 2001, pp. 100-106

Abstract

The detection of specific IgE-antibodies against natural latex (NL in serum by means of the CAP-FEIA-system or the Uni CAP 100-system (Pharmacia & Upjohn) and the AlaSTAT-system are very sensitive and specific methods. However, in some patients it is not possible to detect the specific IgE-antibodies. In 40% of our examinated patients suffering from a clinically relevant allergy against natural latex the specific IgE antibodies couldnt be detected. In addition these serum samples were investigated by means of the immunoblot (DPC Biermann) and the CAST (Buhlmann Distribution by DPC Biermann) using EDTA-blood. Specific IgE-antibodies with bands at 14 kD (Hev b 1; Rubber elongation factor), 16 kD (Hev b5), 22 kD (Hev b 6), 27 kD (class-I-chitinase), 30 kD (Hevamin), 35 kD (Hev b 2), 41-44 kD (Hev b7, Hev b 2)) and at 50-57 kD (Hev b it) and at 60 - 100 kD (latexproteins) could be found in the immunoblots. Moreover, increased sulfidoleukotriene values were found in the Cellular Antigen Stimulation Test. Due to evaluate the CAST and immunoblot investigations as methods for the detection of allergies against latex a sensitivity of 100% and a specificity of 96% for the CAST and for immunoblots 100% sensitivity and specificity could be found compared with the skin tests. However, the calculated values for the UniCAP-system (sensitivity55%, specificity 100%) and the AlaSTAT-system (sensitivity 56%, specificity 96%) are lower. Immunoblot and CAST seems to be a useful completition in diagnosis of allergies against natural latex especially in those cases if the detection of sIgE-antibodies against latex is not possible in the CAP-FEIA-system or other comparable methods.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:33:36