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Titolo:
An inducible packaging cell system for safe, efficient lentiviral vector production in the absence of HIV-1 accessory proteins
Autore:
Pacchia, AL; Adelson, ME; Kaul, M; Ron, Y; Dougherty, JP;
Indirizzi:
Robert Wood Johnson Med Sch, Dept Mol Genet & Microbiol, Piscataway, NJ 08854 USA Robert Wood Johnson Med Sch Piscataway NJ USA 08854 cataway, NJ 08854 USA Rutgers State Univ, Grad Program Microbiol & Mol Genet, New Brunswick, NJ 08903 USA Rutgers State Univ New Brunswick NJ USA 08903 New Brunswick, NJ 08903 USA
Titolo Testata:
VIROLOGY
fascicolo: 1, volume: 282, anno: 2001,
pagine: 77 - 86
SICI:
0042-6822(20010330)282:1<77:AIPCSF>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; VIVO GENE DELIVERY; IN-VIVO; RETROVIRUS VECTORS; MAMMALIAN-CELLS; TRANSGENIC MICE; HIGH-TITER; EXPRESSION; TYPE-1; PSEUDOTRANSDUCTION;
Keywords:
HIV-1; lentiviral vector; cell line; regulated viral gene expression; inducible; ecdysone; GFP; gene therapy; transgene; nondividing cells; HeLa cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Dougherty, JP Robert Wood Johnson Med Sch, Dept Mol Genet & Microbiol, 675Hoes Lane, Piscataway, NJ 08854 USA Robert Wood Johnson Med Sch 675 Hoes Lane Piscataway NJ USA 08854
Citazione:
A.L. Pacchia et al., "An inducible packaging cell system for safe, efficient lentiviral vector production in the absence of HIV-1 accessory proteins", VIROLOGY, 282(1), 2001, pp. 77-86

Abstract

Lentiviral vectors based on human immunodeficiency virus type 1 (HIV-1) possess the ability to deliver exogenous genes to both dividing and nondividing cells and to subsequently establish a stable provirus in these target cells, which can allow long-term expression of the transferred gene. Herein we describe a stable packaging cell line that is devoid of HIV-1 tat, vif, vpr, vpu, and nef. In order to avoid any risk of cytotoxicity associated with constitutive expression of HIV-I protease or the VSV-G envelope protein, transcription of the packaging and envelope constructs was tightly controlled by employing the ecdysone-inducible system. Using this cell line, we have been able to consistently generate concentrated pseudotyped vector virus stocks with titers in the range of 10(8) IU/ml, which can efficiently transduce actively dividing and growth-arrested cells in vitro. This novel packaging cell line for lentiviral vectors facilitates the production of high-titer virus stocks in the absence of replication-competent virus and providesus with an important tool for use in future gene transfer studies. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 03:57:43