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Titolo:
Characterisation and clonal relationships of Shiga-toxigenic Escherichia coli (STEC) isolated from Australian dairy cattle
Autore:
Cobbold, R; Desmarchelier, P;
Indirizzi:
Univ Queensland, Dept Microbiol & Parasitol, St Lucia, Qld 4072, AustraliaUniv Queensland St Lucia Qld Australia 4072 St Lucia, Qld 4072, Australia Food Sci Australia, Tingalpa DC, Qld 4173, Australia Food Sci Australia Tingalpa DC Qld Australia 4173 DC, Qld 4173, Australia
Titolo Testata:
VETERINARY MICROBIOLOGY
fascicolo: 4, volume: 79, anno: 2001,
pagine: 323 - 335
SICI:
0378-1135(20010419)79:4<323:CACROS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIELD GEL-ELECTROPHORESIS; MULTIPLEX PCR; O157-H7; TOXIN; PREVALENCE; STRAINS; WISCONSIN; FARMS; GENES; EAEA;
Keywords:
cattle-bacteria; Escherichia coli 0157 : H7; PFGE; STEC; virulence; Australia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Cobbold, R Univ Queensland, Dept Microbiol & Parasitol, St Lucia, Qld 4072, Australia Univ Queensland St Lucia Qld Australia 4072 ld 4072, Australia
Citazione:
R. Cobbold e P. Desmarchelier, "Characterisation and clonal relationships of Shiga-toxigenic Escherichia coli (STEC) isolated from Australian dairy cattle", VET MICROB, 79(4), 2001, pp. 323-335

Abstract

A total of 136 Shiga toxin-producing Escherichia coli (STEC) isolated during a longitudinal survey of three Australian dairy farms were examined to determine their virulence factors, serotype and genomic relationships. This study aimed to assess the potential of these STEC to cause disease in humans and to analyse the on-farm ecology of STEC. Virulence factors (sis, eae, ehxA) were used as determinants of potential to be enterohaemorrhagic E. coli (EHEC) and were examined using polymerase chain reaction (PCR). Among the cattle groups tested, calves, both before and during weaning, shed the most putative EHEC and were the main source of serotypes commonly associated with human disease. E, coli O157:H7 and E, coli O26:H11 represented 9.4 and7.8% of cattle STEC isolates respectively, with other putative EHEC serotypes reported for the first time from cattle. Based on serotype and virulence factors, 20% of STEC were putative EHEC. Pulsed-field gel electrophoresis(PFGE) was used to compare the genomic profiles of STEC from dairy farms. Isolates common to cattle and the farm environment were identified. Multiple strains of STEC with high clonal turnover were detected in the faeces of cattle, and isolates appeared to be specific to individual farms. To fully assess the pre-slaughter EHEC risk factors on-farm, examination of STEC virulence is as important as determination of STEC prevalence. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 19:37:24