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Titolo:
Low or no antibody responses to human immunodeficiency virus type 1 Nef ininfected carriers with subtype E, in contrast to subtype B that showed antibodies preferentially recognizing subtype-specific Nef epitopes
Autore:
Komoto, S; Kinomoto, M; Ibrahim, MS; Qiu, Z; Auwanit, W; Ayuthaya, PIN; Otake, T; Mori, H; Oishi, I; Kurosu, T; Takahashi, H; Mukai, T; Ikuta, K;
Indirizzi:
Osaka Univ, Microbial Dis Res Inst, Dept Virol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 t Virol, Suita, Osaka 5650871, Japan Hokkaido Univ, Inst Immunol Sci, Sect Serol, Kita Ku, Sapporo, Hokkaido 0600815, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600815 oro, Hokkaido 0600815, Japan Natl Inst Hlth, Dept Med Sci, Immunol Sect, Nonthaburi 11000, Thailand Natl Inst Hlth Nonthaburi Thailand 11000 ect, Nonthaburi 11000, Thailand Osaka Prefectural Inst Publ Hlth, Pathol Lab, Higashinari Ku, Osaka 5370025, Japan Osaka Prefectural Inst Publ Hlth Osaka Japan 5370025 saka 5370025, Japan
Titolo Testata:
VACCINE
fascicolo: 20-22, volume: 19, anno: 2001,
pagine: 3019 - 3032
SICI:
0264-410X(20010406)19:20-22<3019:LONART>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOTOXIC T-LYMPHOCYTES; LONG-TERM SURVIVOR; HIV-1 NEF; DISEASE PROGRESSION; DOWN-REGULATION; MONOCLONAL-ANTIBODIES; LYMPHOID ORGANS; CD4 LYMPHOCYTES; IN-VITRO; PROTEIN;
Keywords:
HIV-1; immune response; Nef; Env; Gag; subtype E; subtype B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
75
Recensione:
Indirizzi per estratti:
Indirizzo: Mukai, T Osaka Univ, Microbial Dis Res Inst, Dept Virol, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 5650871, Japan
Citazione:
S. Komoto et al., "Low or no antibody responses to human immunodeficiency virus type 1 Nef ininfected carriers with subtype E, in contrast to subtype B that showed antibodies preferentially recognizing subtype-specific Nef epitopes", VACCINE, 19(20-22), 2001, pp. 3019-3032

Abstract

The viral accessory gene product Nef has been shown to play an important role in human immunodeficiency virus type 1 (HIV-1)-induced pathogenesis. Only little information is available regarding the differences in the host immune responses against Nef protein and its function in vivo among differentsubtypes of HIV-1. In the present study, we showed marked differences in the immune responses to Nef protein between subtypes B and E. The amino acidsequence in subtype E Nef showed 72% homology with that in subtype B. Mostmurine monoclonal antibodies obtained by immunization with subtype B or E Nef protein showed cross-reactivity with both Nef proteins (80 and 67%, respectively). Next, we focused on the immune responses among infected Japanese and Thai individuals. Subtyping of the individuals into B and E was carried out by enzyme-linked immunosorbent assay (ELISA) using synthetic peptides corresponding to the V3 loop representing the principal neutralizing domain. Most of the sera from these individuals reacted strongly with Gag p24 proteins derived from subtypes B and E at similar levels. However, the: immune responses among these individuals to Nef protein were markedly different. Some subtype B-infected Japanese and Thai individuals (40 and 35%, respectively) showed higher levels of anti-Nef antibodies, although these antibodies preferentially recognized epitopes specific to subtype B. On the other hand, most of the subtype E-infected Japanese and Thai individuals showed low or no antibody responses to Nef proteins. Thus, immune responses to Nef were markedly different between subtypes B- and E-infected carriers, suggesting different function(s) for Nef in AIDS pathogenesis. Further, vaccine design must take into account the different subtypes of HIV-1, (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 22:13:05