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Titolo:
Enhanced immune responses to viral epitopes by combining macrophage-inducible expression with multimeric display on a Salmonella vector
Autore:
Chen, HQ; Schifferli, DM;
Indirizzi:
Univ Penn, Sch Vet Med, Dept Pathobiol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Pathobiol, Philadelphia, PA 19104 USA
Titolo Testata:
VACCINE
fascicolo: 20-22, volume: 19, anno: 2001,
pagine: 3009 - 3018
SICI:
0264-410X(20010406)19:20-22<3009:EIRTVE>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSMISSIBLE GASTROENTERITIS VIRUS; ENTEROTOXIGENIC ESCHERICHIA-COLI; ATTENUATED SALMONELLA; VACCINE STRAIN; MONOCLONAL-ANTIBODIES; HETEROLOGOUS ANTIGEN; PROTECTIVE IMMUNITY; STABLE MAINTENANCE; ORAL VACCINATION; MUCOSAL IMMUNITY;
Keywords:
fimbriae; TGEV; Salmonella typhimurium; vaccine; pagC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Schifferli, DM Univ Penn, Sch Vet Med, Dept Pathobiol, 3800 Spruce St, Philadelphia, PA 19104 USA Univ Penn 3800 Spruce St Philadelphia PA USA 19104 9104 USA
Citazione:
H.Q. Chen e D.M. Schifferli, "Enhanced immune responses to viral epitopes by combining macrophage-inducible expression with multimeric display on a Salmonella vector", VACCINE, 19(20-22), 2001, pp. 3009-3018

Abstract

In this study, the immunogenicity of chimeric 987P fimbriae on a Salmonella vaccine strain was improved by optimizing fimbrial expression. The constitutive tetA promoter and the in vivo activated nirB and pagC promoters wereevaluated for their use to express two epitopes of the transmissible gastroenteritis virus (TGEV) spike protein carried by fimbriae which were displayed on a Salmonella vaccine strain. Constructs with the pagC promoter were shown to drive increased expression of chimeric 987P fimbriae in macrophages as well as in Mg2+ -poor media, mimicking a major environmental signal found in Salmonella-containing endocytic vacuoles of macrophages. Mice immunized orally with a Salmonella vaccine strain which expressed chimeric fimbriae from the pagC promoter elicited significantly higher mucosal and systemic immune responses to both the 987P fimbriae and the TGEV epitopes than mice immunized with the same strain hosting a tetA or nirB promoter-driven expression plasmid. Moreover, only the Salmonella vaccine strains harboring a plasmid with the pagC promoter, with or without an additional tetA promoterin tandem, elicited neutralizing antibodies to TGEV. This indicated that the pagC promoter can be used successfully to improve epitope-display by chimeric fimbriae on Salmonella vaccine strains for the induction of a desiredimmune response. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:20:39