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Titolo:
Qualitative and quantitative molecular detection of enteroviruses in waterfrom bathing areas and from a sewage treatment plant
Autore:
Schvoerer, E; Ventura, M; Dubos, O; Cazaux, G; Serceau, R; Gournier, N; Dubois, V; Caminade, P; Fleury, HJA; Lafon, ME;
Indirizzi:
Univ Bordeaux 2, Virol Lab, F-33076 Bordeaux, France Univ Bordeaux 2 Bordeaux France F-33076 ol Lab, F-33076 Bordeaux, France Univ Bordeaux 2, CNRS, Lab Replicat & Express Genomes Eucaryotes & Retro, F-33076 Bordeaux, France Univ Bordeaux 2 Bordeaux France F-33076 Retro, F-33076 Bordeaux, France Terrasse Marechal Koenig, Direct Departementale Affaires Sanitaires & Socia, F-33062 Bordeaux, France Terrasse Marechal Koenig Bordeaux France F-33062 -33062 Bordeaux, France Inst Europeen Environm Bordeaux, F-33000 Bordeaux, France Inst Europeen Environm Bordeaux Bordeaux France F-33000 Bordeaux, France
Titolo Testata:
RESEARCH IN MICROBIOLOGY
fascicolo: 2, volume: 152, anno: 2001,
pagine: 179 - 186
SICI:
0923-2508(200103)152:2<179:QAQMDO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; HEPATITIS-A VIRUS; REVERSE-TRANSCRIPTASE PCR; SEMINESTED PCR; VIRAL GASTROENTERITIS; ENTERIC VIRUSES; DRINKING-WATER; CELL-CULTURE; OUTBREAK; SHELLFISH;
Keywords:
enteroviruses; environmental waters; indicator; quantitative RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Lafon, ME Univ Bordeaux 2, Virol Lab, 146 Rue Leo Saignat, F-33076 Bordeaux, France Univ Bordeaux 2 146 Rue Leo Saignat Bordeaux France F-33076 nce
Citazione:
E. Schvoerer et al., "Qualitative and quantitative molecular detection of enteroviruses in waterfrom bathing areas and from a sewage treatment plant", RES MICROB, 152(2), 2001, pp. 179-186

Abstract

Pathogenic enteric viruses can be introduced into the environment as a result of human activities. Enteroviruses are regularly detected in environmental waters or shellfish and can provoke potentially serious diseases. Some authors believe that enteroviruses could represent an interesting indicatorof Viral contamination in the environment. Since molecular approaches seemto be promising for the detection of these viruses, we developed a simple qualitative RT-PCR procedure for enteroviruses, together with a quantitative RT-PCR assay using RNA internal standard. After one-tube-RT-PCR, this standard and wild enterovirus RNA were detected by differential hybridization with specific probes and a fluorimetric reaction. The quantification of enteroviruses, conducted in a sewage treatment plant, showed a decreasing number of genomic copies from the entrance to the exit (from 3.8 x 10(5) to 5.4x 10(4) RNA copies/mL) but indicated the presence of enterovirus RNA in the neighboring river (2.2 x 10(3) RNA copies/mL). In bathing areas, enterovirus RNA was detected in 16 out of 226 samples, with copies numbers ranging from 3.7 x 10(2) RNA copies/mL to 7 x 10(4) RNA copies/mL. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:24:45