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Titolo:
Molecular cloning and functional characterization of two kinds of betaine-aldehyde dehydrogenase in betaine-accumulating mangrove Avicennia marina (Forsk.) Vierh.
Autore:
Hibino, T; Meng, YL; Kawamitsu, Y; Uehara, N; Matsuda, N; Tanaka, Y; Ishikawa, H; Baba, S; Takabe, T; Wada, K; Ishii, T; Takabe, T;
Indirizzi:
Meijo Univ, Fac Sci & Technol, Tenpaku Ku, Nagoya, Aichi 4688502, Japan Meijo Univ Nagoya Aichi Japan 4688502 ku Ku, Nagoya, Aichi 4688502, Japan Meijo Univ, Res Inst, Tenpaku Ku, Nagoya, Aichi 4688502, Japan Meijo UnivNagoya Aichi Japan 4688502 ku Ku, Nagoya, Aichi 4688502, Japan Univ Ryukyus, Fac Agr, Okinawa 9030213, Japan Univ Ryukyus Okinawa Japan9030213 kyus, Fac Agr, Okinawa 9030213, Japan Nagoya Univ, Grad Sch Agr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 a Ku, Nagoya, Aichi 4648601, Japan Kanazawa Univ, Fac Sci, Kanazawa, Ishikawa 92011, Japan Kanazawa Univ Kanazawa Ishikawa Japan 92011 nazawa, Ishikawa 92011, Japan Forestry & Prod Res Inst, Tsukuba, Ibaraki 4648601, Japan Forestry & Prod Res Inst Tsukuba Ibaraki Japan 4648601 aki 4648601, Japan
Titolo Testata:
PLANT MOLECULAR BIOLOGY
fascicolo: 3, volume: 45, anno: 2001,
pagine: 353 - 363
SICI:
0167-4412(200102)45:3<353:MCAFCO>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCINE BETAINE; CHOLINE MONOOXYGENASE; ESCHERICHIA-COLI; OSMOTIC-STRESS; ABSCISIC-ACID; CDNA CLONING; SUGAR-BEET; EXPRESSION; PLANTS; TOLERANCE;
Keywords:
Avicennia marina; betaine; betaine aldehyde dehydrogenase; mangrove; salt tolerance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Takabe, T Meijo Univ, Fac Sci & Technol, Tenpaku Ku, Nagoya, Aichi 4688502, Japan Meijo Univ Nagoya Aichi Japan 4688502 oya, Aichi 4688502, Japan
Citazione:
T. Hibino et al., "Molecular cloning and functional characterization of two kinds of betaine-aldehyde dehydrogenase in betaine-accumulating mangrove Avicennia marina (Forsk.) Vierh.", PLANT MOL B, 45(3), 2001, pp. 353-363

Abstract

Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could bedetected only in Avicennia marina. Pinitol was the main osmoprotectant in the other three species. The level of glycinebetaine in A. marina increasedunder high salinity. Betaine-aldehyde dehydrogenase (BADH) was detected inall four species, but choline monooxygenase could not be detected. A cDNA library was constructed from the leaves of A. marina. Two kinds of BADH cDNAs were isolated, one homologous to the spinach chloroplast BADH, and the other with unique residues SKL at the end of C-terminus. The BADH transcription levels of the former were higher than those of the latter. The levels of the former BADH increased at high salinity whereas those of the latter were independent of salinity. BADHs were expressed in Escherichia coli and purified. Two kinds of A. marina BADHs exhibited similar kinetic and stability properties, but were significantly different from those of spinach BADH. A. marina BADHs efficiently catalyzed the oxidation of betainealdehyde, butnot the oxidation of omega -aminoaldehydes and were more stable at high temperature than the spinach BADH.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/10/20 alle ore 21:12:05