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Titolo:
Effects of neurotensin on discharge rates of rat suprachiasmatic nucleus neurons in vitro
Autore:
Coogan, AN; Rawlings, N; Luckman, SM; Piggins, HD;
Indirizzi:
Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT M13 9PT, Lancs, England Univ London Kings Coll, London WC2R 2LS, England Univ London Kings Coll London England WC2R 2LS London WC2R 2LS, England
Titolo Testata:
NEUROSCIENCE
fascicolo: 3, volume: 103, anno: 2001,
pagine: 663 - 672
SICI:
0306-4522(2001)103:3<663:EONODR>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; NEUROPEPTIDE-Y; SUBSTANTIA-NIGRA; INTERGENICULATE LEAFLET; RECEPTOR ANTAGONIST; CIRCADIAN-RHYTHMS; MESSENGER-RNA; BRAIN-SLICES; HAMSTER; SYSTEM;
Keywords:
neurotensin; suprachiasmatic nucleus; circadian; electrophysiology; pharmacology; brain slice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Piggins, HD Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT ncs, England
Citazione:
A.N. Coogan et al., "Effects of neurotensin on discharge rates of rat suprachiasmatic nucleus neurons in vitro", NEUROSCIENC, 103(3), 2001, pp. 663-672

Abstract

The neuropeptide neurotensin and two classes of its receptors, the neurotensin receptor-1 and 2, are present in the suprachiasmatic nucleus of the mammalian hypothalamus. The suprachiasmatic nucleus houses the mammalian central circadian pacemaker, but the effects of neurotensin on cellular activity in this circadian pacemaker are unknown. In this study, we examined the effects of neurotensin on the spontaneous discharge rate of rat SCN cells inan in vitro slice preparation. Neurotensin (1-10 muM) increased cell firing rate in similar to 50% of cells tested, while similar to 10% of suprachiasmatic cells tested showed a decrease in firing rate in response to neurotensin. These effects of neurotensin were not altered by the GABA receptor antagonist bicuculline (20 muM) or the glutamate receptor antagonists, D-aminophosphopentanoic acid (50 muM) and 6-cyano-7-nitroquinoxaline-2,3-dione (20 muM). The neurotensin receptor selective antagonists SR48692 and SR142948a (10 muM) failed to antagonise neurotensin responses in the majority of cells examined. Compounds that function as agonists selective for the neurotensin-receptor subtypes 1 and 2, JMV-510 and JMV-431 respectively, elicited neurotensin-like responses in similar to 90% of cells tested. Six out of seven cells tested responded to both JMV-510 and JMV-431. Neuropeptide Y (100nM) treatment of suprachiasmatic nucleus slices was found to elicit profound suppression of neuronal firing rate. Go-application of neurotensin with neuropeptide Y significantly (P < 0.05) reduced the duration of the response, as compared to that elicited with neuropeptide Y alone. Together, these results demonstrate for the first time the actions of neurotensin in the suprachiasmatic nucleus and raise the possibility that this neuropeptide may play a role in modulating circadian pacemaker function. <(c)> 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:07:13