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Titolo:
A new 'online' method to measure increased exhaled isoprene in end-stage renal failure
Autore:
Davies, S; Spanel, P; Smith, D;
Indirizzi:
Univ Keele, Postgrad Med Sch, Ctr Sci & Technol Med, Stoke On Trent ST4 7QB, Staffs, England Univ Keele Stoke On Trent Staffs England ST4 7QB ST4 7QB, Staffs, England N Staffordshire Hosp, Dept Nephrol, Stoke On Trent, Staffs, England N Staffordshire Hosp Stoke On Trent Staffs England rent, Staffs, England Acad Sci Czech Republ, J Heyrovsky Inst Phys Chem, CR-18223 Prague 8, Czech Republic Acad Sci Czech Republ Prague Czech Republic 8 3 Prague 8, Czech Republic
Titolo Testata:
NEPHROLOGY DIALYSIS TRANSPLANTATION
fascicolo: 4, volume: 16, anno: 2001,
pagine: 836 - 839
SICI:
0931-0509(200104)16:4<836:AN'MTM>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ION FLOW TUBE; TRACE GAS-ANALYSIS; BREATH ISOPRENE; QUANTITATIVE-ANALYSIS; MASS-SPECTROMETRY; EXPIRED AIR; PENTANE; CHROMATOGRAPHY; EXCRETION; PLASMA;
Keywords:
biocompatibility; breath analysis; haemodialysis; isoprene; SIFT-MS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Davies, S N Staffordshire Hosp Trust, Dept Nephrol, Princes Rd, Stoke On Trent ST4 7LN, Staffs, England N Staffordshire Hosp Trust Princes Rd Stoke On Trent Staffs England ST4 7LN
Citazione:
S. Davies et al., "A new 'online' method to measure increased exhaled isoprene in end-stage renal failure", NEPH DIAL T, 16(4), 2001, pp. 836-839

Abstract

Background. Isoprene is the most abundant hydrocarbon present in breath, and recent reports indicate that breath concentrations increase following haemodialysis. The purpose of this study was to establish whether selected ion flow tube mass spectrometry (SIFT-MS), a newly established technique in breath analysis, may be used to quantify breath isoprene in haemodialysis patients in the clinical setting. SIFT-MS is compared and contrasted with theestablished gas chromatography mass spectrometric technique for this purpose. Methods. Three consecutive exhalations from 19 haemodialysis patients (12 males, seven females) undergoing a morning dialysis shift were analysed just prior to commencing treatment. Within 5 min of completing their usual dialysis regimen, using polysulphone membranes, the breath of each patient wasanalysed again. Additional contemporary samples were obtained from 17 normal controls. Breath isoprene was quantified using SIFT-MS, a method previously validated quantitatively using neat isoprene. Results. Successful measurements of breath isoprene were obtained for eachsubject within 2 min, with minimum disruption to a busy dialysis environment. The coefficient of variation of triplicate measurements of breath isoprene was < 10%. Prior to dialysis, the mean (+/- SD) breath isoprene concentration (138 +/- 63 parts per billion (ppb)) was significantly greater than for normal controls (89 +/- 36 ppb; P = 0.016). Immediately following treatment, breath isoprene increased significantly to 184 +/- 95 ppb (P = 0.023). Conclusions. SIFT-MS permits the accurate and rapid measurement of breath isoprene in haemodialysis patients in the clinical setting. The previously reported increase in breath isoprene following dialysis treatment is confirmed. SIFT-MS is the ideal analytical tool to investigate this phenomenon further.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 03:01:23