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Titolo:
Renal osteopontin protein and mRNA upregulation during acute nephrotoxicity in the rat
Autore:
Verstrepen, WA; Persy, VP; Verhulst, A; Dauwe, S; De Broe, ME;
Indirizzi:
Univ Antwerp, Dept Nephrol Hypertens, B-2650 Edegem, Belgium Univ AntwerpEdegem Belgium B-2650 rol Hypertens, B-2650 Edegem, Belgium
Titolo Testata:
NEPHROLOGY DIALYSIS TRANSPLANTATION
fascicolo: 4, volume: 16, anno: 2001,
pagine: 712 - 724
SICI:
0931-0509(200104)16:4<712:ROPAMU>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERSTITIAL FIBROSIS; UP-REGULATION; IN-VITRO; EXPRESSION; KIDNEY; INJURY; ISCHEMIA; DISEASE; GLOMERULONEPHRITIS; MIGRATION;
Keywords:
acute renal failure; gentamicin; kidney; mercuric chloride;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: De Broe, ME Univ Antwerp, Dept Nephrol Hypertens, P-A Univ Hosp Antwerp,Wilrijkstr 10,B-2650 Edegem, Belgium Univ Antwerp P-A Univ Hosp Antwerp,Wilrijkstr 10 Edegem Belgium B-2650
Citazione:
W.A. Verstrepen et al., "Renal osteopontin protein and mRNA upregulation during acute nephrotoxicity in the rat", NEPH DIAL T, 16(4), 2001, pp. 712-724

Abstract

Background. The effect of segment-specific proximal tubular injury on spatio-temporal osteopontin (OPN) distribution was determined in two different nephrotoxic rat models to evaluate its conceivability with a possible role for OPN in acute renal failure (ARF). OPN gene expression was further determined in proximal and distal tubular cells to investigate the origin of increased renal OPN. Methods. Renal OPN protein and mRNA expression were compared in the rat during mercuric-chloride- vs gentamicin-induced ARF using immunohistochemistry and ill situ hybridization. Results. Mercuric chloride primarily induced tubular injury and subsequentcell proliferation in proximal straight tubules (PST), whereas gentamicin predominantly injured proximal convoluted tubules (PCT). In both models, the distribution of OPN protein was associated with increased OPN mRNA levelsin proximal as well as distal tubular cells. However, upregulation was delayed in the proximal tubular segment suffering most from injury, i.e. PCT in gentamicin ARF vs PST in mercuric-chloride ARF. OPN immunostaining at theapical cell membrane from distal tubules was in contrast to perinuclear vesicular staining in proximal tubular cells. Conclusions. OPN gene and protein expression is induced in both proximal and distal tubular cells during rat toxic ARF. The distinct subcellular localization in proximal vs distal tubular cells indicates differences in OPN processing and/or handling. The spatiotemporal distribution is consistent with a possible role ill renal injury and regeneration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:46:39