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Titolo:
Identification of cellular origin of type I collagen in glomeruli of rats with crescentic glomerulonephritis induced by anti-glomerular basement membrane antibody
Autore:
He, JS; Hayashi, K; Horikoshi, S; Funabiki, K; Shirato, I; Tomino, Y;
Indirizzi:
Juntendo Univ, Sch Med, Dept Med, Div Nephrol,Bunkyo Ku, Tokyo 1138421, Japan Juntendo Univ Tokyo Japan 1138421 ephrol,Bunkyo Ku, Tokyo 1138421, Japan
Titolo Testata:
NEPHROLOGY DIALYSIS TRANSPLANTATION
fascicolo: 4, volume: 16, anno: 2001,
pagine: 704 - 711
SICI:
0931-0509(200104)16:4<704:IOCOOT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; EXPRESSION; GLOMERULOSCLEROSIS; PROGRESSION; NEPHRITIS; DISEASE; CELLS;
Keywords:
crescentic glomerulonephritis; cytokeratin; in situ hybridization; parietal epithelial cell; type I collagen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Tomino, Y Juntendo Univ, Sch Med, Dept Med, Div Nephrol,Bunkyo Ku, 2-1-1 Hongo, Tokyo 1138421, Japan Juntendo Univ 2-1-1 Hongo Tokyo Japan 1138421 yo 1138421, Japan
Citazione:
J.S. He et al., "Identification of cellular origin of type I collagen in glomeruli of rats with crescentic glomerulonephritis induced by anti-glomerular basement membrane antibody", NEPH DIAL T, 16(4), 2001, pp. 704-711

Abstract

Background. Type I collagen is an interstitial collagen, which is not present in normal glomeruli. As type I collagen was observed in advanced glomerular lesions, it appears to be associated with deterioration of renal function. However, the origins of cells expressing type I collagen mRNA in glomeruli of diseased kidneys remains controversial. Methods. We examined the expression of type I collagen in glomeruli at protein and mRNA levels in rat crescentic glomerulonephritis induced by anti-glomerular basement membrane (GBM) antibody. In addition, in situ hybridization and immunohistochemical staining of serial sections were performed to identify the cellular origin of type I collagen in glomeruli. Results. Semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR) in isolated glomeruli showed that mRNA expression of type I collagen was remarkably increased on days 7, 14, and 28 after anti-GBM antibody injection (12.2+/-1.4, 20.2+/-2.1 and 14.6+/-1.0-fold over day 0, respectively). Immunofluorescence for type I collagen demonstrated marked staining in the fibrocellular and fibrous crescents, and weak staining within glomerular mesangial areas. In close association with mRNA levels analysed by RT-PCR, in situ hybridization revealed predominant presence of alpha1(I) collagen mRNA in cells within crescentic areas and Bowman's capsules. Serial section analysis for immunostaining and in situ hybridization showed that some alpha1(I) collagen mRNA-positive cells were also positive for cytokeratin. In contrast, no alpha1(I) collagen mRNA-positive cells were stained by ED-1and podocalyxin. Conclusions. It appears that increased expression of type I collagen at the protein and mRNA levels in glomeruli is involved in the progression of glomerulonephritis. At least in this crescentic model, parietal epithelial cells (PECs) may partially contribute to the dysregulated production of type I collagen, which leads to glomerulosclerosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:31:51