Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Prostasome-like particles are involved in the transfer of P25b from the bovine epididymal fluid to the sperm surface
Autore:
Frenette, G; Sullivan, R;
Indirizzi:
Univ Laval, Fac Med, Ctr Rech Biol Reprod, St Foy, PQ G1K 7P4, Canada UnivLaval St Foy PQ Canada G1K 7P4 ol Reprod, St Foy, PQ G1K 7P4, Canada Univ Laval, Fac Med, Dept Obstet Gynecol, St Foy, PQ G1K 7P4, Canada Univ Laval St Foy PQ Canada G1K 7P4 t Gynecol, St Foy, PQ G1K 7P4, Canada
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 1, volume: 59, anno: 2001,
pagine: 115 - 121
SICI:
1040-452X(200105)59:1<115:PPAIIT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEMINAL PLASMA; PROTEIN; HAMSTER; SPERMATOZOA; MEMBRANE; ANTIGEN; P26H; RAT; GLYCOPROTEIN; MATURATION;
Keywords:
prostasome-like particles; bovine epididymis; P25b; sperm maturation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Sullivan, R CHU Laval, Ctr Rech, Unite Ontogenie Reprod, 2705 Blvd Laurier, St Foy, PQG1V 4G2, Canada CHU Laval 2705 Blvd Laurier St Foy PQ Canada G1V 4G2 2, Canada
Citazione:
G. Frenette e R. Sullivan, "Prostasome-like particles are involved in the transfer of P25b from the bovine epididymal fluid to the sperm surface", MOL REPROD, 59(1), 2001, pp. 115-121

Abstract

In bulls, P25b is a sperm protein associated with the plasma membrane covering the acrosome. The amount of P25b bound to a constant number of spermatozoa varies from one individual to the other, low levels being associated with bull subfertility. In this study, we describe the epididymal origin of P25b using Western blot analysis. Whereas P25b was undetectable on caput spermatozoa, the amount of P25b associated to a constant number of spermatozoa increases from the corpus to the cauda. Prostasome-like particles were prepared by ultracentrifugation of epididymal fluid. P25b appears to be also associated with those membranous vesicles in increasing amounts a long the epididymis. P25b is anchored to the plasma membrane of spermatozoa through glycosylphosphatidyl-inositol as shown by the ability of phospholipase C, but not of high salt treatment, to release P25b. Coincubation experiments revealed that prostasome-like particles are able to transfer P25b to spermatozoa, this process being more efficient at slightly acidic pH. P25b thus appears to be a marker of sperm epididymal maturation in bulls. Mol. Reprod. Dev. 59:115-121, 2001. (C) 2001. Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:29:35