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Titolo:
Mitogen activated protein kinase plays a significant role in metaphase II arrest, spindle morphology, and maintenance of maturation promoting factor activity in bovine oocytes
Autore:
Gordo, AC; He, CL; Smith, S; Fissore, RA;
Indirizzi:
Univ Massachusetts, Dept Vet & Anim Sci, Amherst, MA 01003 USA Univ Massachusetts Amherst MA USA 01003 & Anim Sci, Amherst, MA 01003 USA Univ Porto, Inst Ciencias Biomed Abel Salazar, P-4100 Porto, Portugal UnivPorto Porto Portugal P-4100 ed Abel Salazar, P-4100 Porto, Portugal
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 1, volume: 59, anno: 2001,
pagine: 106 - 114
SICI:
1040-452X(200105)59:1<106:MAPKPA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERMINAL VESICLE BREAKDOWN; CLEAVING XENOPUS-EMBRYOS; MAP KINASE; MOUSE OOCYTES; MEIOTIC MATURATION; CYCLIN-B; M-PHASE; MICROTUBULE DYNAMICS; PORCINE OOCYTES; POTENTIAL ROLE;
Keywords:
mammalian eggs; mos; oocyte maturation; MKP-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Fissore, RA Univ Massachusetts, Dept Vet & Anim Sci, Amherst, MA 01003 USAUniv Massachusetts Amherst MA USA 01003 Amherst, MA 01003 USA
Citazione:
A.C. Gordo et al., "Mitogen activated protein kinase plays a significant role in metaphase II arrest, spindle morphology, and maintenance of maturation promoting factor activity in bovine oocytes", MOL REPROD, 59(1), 2001, pp. 106-114

Abstract

Mammalian oocytes are arrested at the G2/M transition of the first meioticdivision from which, after reaching full size and subsequent to an LH surge, they undergo final maturation. Oocyte maturation, which involves germinal vesicle breakdown, progression through metaphase I (MI), and arrest at Mil, is triggered and regulated by the coordinated action of two kinases, maturation promoting factor (MPF) and mitogen activated protein kinase (MAPK). The importance of the role of MPF in mammalian oocyte maturation is well established, while the role of MAPK, although well understood in mouse oocytes, has not been fully elucidated in oocytes of targe domestic species, especially bovine oocytes. Here we show that injection of MKP-1 mRNA, which encodes a dual specificity MAPK phosphatase, into germinal vesicle stage bovine oocytes prevents the activation of MAPK during maturation. Despite the lack of MAPK activity, MKP-1-injected oocytes resume and progress through meiosis, although they are unable to arrest at Mit stage and, by 22-26-hour post-maturation, exhibit decondensed pronucleus-like chromatin, a clear signof parthenogenetic activation. MKP-1-injected bovine oocytes exhibit normal activation of MPF activity; however, by 18-hour post-maturation, MPF activity starts to decline and by 22-26 hr MPF activity is absent. MKP-1-injected oocytes also show disorganized MII spindles with poorly aligned chromosomes. In summary, our results demonstrate that in bovine oocytes MAPK activity is required for MII arrest, maintenance of MPF activity, and spindle organization. Mol. Reprod. Dev. 59:106-114, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 20:50:00