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Titolo:
Progesterone induces activation in Octopus vulgaris spermatozoa
Autore:
Tosti, E; Di Cosmo, A; Cuomo, A; Di Cristo, C; Gragnaniello, G;
Indirizzi:
Stn Zool, Dept Cell Biol, I-80121 Naples, Italy Stn Zool Naples Italy I-80121 ool, Dept Cell Biol, I-80121 Naples, Italy Univ Naples, Dept Zool, I-80134 Naples, Italy Univ Naples Naples Italy I-80134 aples, Dept Zool, I-80134 Naples, Italy
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 1, volume: 59, anno: 2001,
pagine: 97 - 105
SICI:
1040-452X(200105)59:1<97:PIAIOV>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPERM ACROSOME REACTION; SEA-URCHIN SPERM; STEROID-HORMONES; CALCIUM INFLUX; ZONA-PELLUCIDA; MOUSE SPERM; EXOCYTOSIS; MOLECULES; RECEPTOR; A23187;
Keywords:
sperm activation; acrosome reaction; cephalopods; reproduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Tosti, E Stn Zool, Dept Cell Biol, I-80121 Naples, Italy Stn Zool NaplesItaly I-80121 Cell Biol, I-80121 Naples, Italy
Citazione:
E. Tosti et al., "Progesterone induces activation in Octopus vulgaris spermatozoa", MOL REPROD, 59(1), 2001, pp. 97-105

Abstract

The purpose of the present study was to determine whether Octopus vulgarisspermatozoa are activated by progesterone stimulation. Spermatozoa were collected from the spermatophores in the Needham's sac of the male (MS) and from the spermathecae of oviducal glands of the female (FS). We used transmission (TEM) and scanning (SEM) electron microscopy to study the morphology of untreated, Ca2+ ionophore A23187 and progesterone-treated MS spermatozoa, and untreated FS spermatozoa. We showed that ionophore and progesterone stimulation of MS spermatozoa induce breakdown of the membranes overlapping the acrosomal region, exposing the spiralized acrosome. These modificationsresemble the acrosome reaction observed in other species. FS stored in thespermathecae did not show the membranes covering the acrosomal region present in the MS spermatozoa. When ionophore and progesterone treatments were performed in Ca2+-iree artificial sea water, no changes were observed, suggesting the role of external calcium in modifying membrane morphology. Lectin studies showed a different fluorescence distribution and membrane arrangement of FS-untreated spermatozoa with respect to the MS, suggesting that spermatozoa transferred in the female genital tract after mating, are stored in a pre-activated state. The plasma membrane of the untreated MS and FS spermatozoa was labelled with Progesterone-BSA-FITC, indicating the presence of plasma membrane progesterone receptor. Taken together these data suggestthat progesterone induces an acrosome-like reaction in MS spermatozoa similar to that induced by calcium elevation. In addition progesterone may playa role in the pre-activation of spermatozoa stored in the female tract, further supporting the hypothesized parallelism between cephalopods and vertebrates. Mol. Reprod. Dev. 59:97-105, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 10:24:40