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Titolo:
Full term development of rabbit oocytes fertilized by intracytoplasmic sperm injection
Autore:
Deng, MQ; Yang, XZJ;
Indirizzi:
Univ Connecticut, Ctr Biotechnol, Dept Anim Sci, Storrs, CT 06269 USA UnivConnecticut Storrs CT USA 06269 Dept Anim Sci, Storrs, CT 06269 USA
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 1, volume: 59, anno: 2001,
pagine: 38 - 43
SICI:
1040-452X(200105)59:1<38:FTDORO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO FERTILIZATION; MEDIATED GENE-TRANSFER; BOVINE OOCYTES; ROUND SPERMATIDS; MOUSE OOCYTES; IMPROVES FERTILIZATION; HAMSTER EGGS; ACTIVATION; CALCIUM; NUCLEI;
Keywords:
ICSI; rabbit; microinjection; oocyte activation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Yang, XZJ Univ Connecticut, Ctr Biotechnol, Dept Anim Sci, 1390 Storrs Rd,U-4163, Storrs, CT 06269 USA Univ Connecticut 1390 Storrs Rd,U-4163 Storrs CT USA 06269 9 USA
Citazione:
M.Q. Deng e X.Z.J. Yang, "Full term development of rabbit oocytes fertilized by intracytoplasmic sperm injection", MOL REPROD, 59(1), 2001, pp. 38-43

Abstract

Intracytoplasmic sperm injection (ICSI) has been applied successfully in the treatment of male infertility in humans and in fertilization research inmice. However, the technique has had limited success in producing offspring in other species including the rabbit. The aim of this research was to test the in vitro and in vivo developmental of rabbit oocytes after ICSI. Sperm used for ICSI were collected from mature Dutch Belted buck and washed 2-3 times with PBS+0.1% polyvinyl alcohol (PVA) and then mixed with 10% polyvinyl pyrrolidone (PVP) prior to microinjection. Oocytes were collected fromsuperovulated does 14-15 hr after hCG injection and were fertilized by microinjection of a single sperm into the ooplasm of each oocyte without additional activation treatment. After ICSI, the presumed zygotes were either cultured in KSOM+10.3% BSA for 4 days or transferred into oviducts of recipient does at the pronuclear or 2-cell stage. A high percentage of fertilization (78%, n=114) and blastocyst development (39%) was obtained after ICSI. Control oocytes, receiving a sham injection, exhibited a lower activation rate (31%, n=51) and were unable to develop to the blastocyst stage, suggesting that the blastocysts developed following ICSI were derived from successful fertilization rather than parthenogenetic development. A total of 113 embryos were transferred to six recipient does. Two recipients became pregnant and delivered seven live young. Our results demonstrated that rabbit oocytes can be successfully fertilized and activated by ICSI and can result in the birth of live offspring. Mol Reprod. Dev. 59:38-43, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:05:35