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Titolo:
In vivo gene transfer to dopamine neurons of rat substantia nigra via the high-affinity neurotensin receptor
Autore:
Alvarez-Maya, I; Navarro-Quiroga, I; Meraz-Rios, MA; Aceves, J; Martinez-Fong, D;
Indirizzi:
Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Fisiol Biofis & Neurociencias, Mexico City 07000, DF, Mexico Inst Politecn Nacl Mexico City DF Mexico 07000 ico City 07000, DF, Mexico Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Biol Celular, Mexico City 07000, DF, Mexico Inst Politecn Nacl Mexico City DF Mexico 07000ico City 07000, DF, Mexico Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Biomed Mol, Mexico City 07000, DF, Mexico Inst Politecn Nacl Mexico City DF Mexico 07000 ico City 07000, DF, Mexico
Titolo Testata:
MOLECULAR MEDICINE
fascicolo: 3, volume: 7, anno: 2001,
pagine: 186 - 192
SICI:
1076-1551(200103)7:3<186:IVGTTD>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLY-L-LYSINE; IN-VIVO; BINDING-SITES; MESSENGER-RNA; MOUSE-BRAIN; DELIVERY; CELLS; VECTOR; POLYLYSINE; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Martinez-Fong, D Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, DeptFisiol Biofis & Neurociencias, Apartado Postal 14-740, Mexico City 07000, DF, Mexico Inst Politecn Nacl Apartado Postal 14-740 Mexico City DF Mexico 07000
Citazione:
I. Alvarez-Maya et al., "In vivo gene transfer to dopamine neurons of rat substantia nigra via the high-affinity neurotensin receptor", MOL MED, 7(3), 2001, pp. 186-192

Abstract

Background: Recently, we synthesized a nonviral gene vector capable of transfecting cell lines taking advantage of neurotensin (NT) internalization. The vector is NT cross-linked with poly-L-lysine, to which a plasmid DNA was bound to form a complex (NT-polyplex). Nigral dopamine neurons are able to internalize NT, thus representing a target for gene transfer via NT-polyplex. This hypothesis was tested here using reporter genes encoding green fluorescent protein or chloramphenicol acetyl transferase. Materials and Methods. NT-polyplex was injected into the substantia nigra. Double immunofluorescence labeling was used to reveal the cell type involved in the propidium iodide-labeled polyplex internalization and reporter gene expression. Results. Polyplex internalization was observed within dopamine neurons butnot within glial cells, and was pre-vented by both hypertonic sucrose solution and SR-48692, a selective nonpeptide antagonist of NT receptors. Reporter gene expression was observed in dopamine neurons from 48 hr up to 15 days after NT-polyplex injection, and was prevented by SR-48692. However, no expression was seen when the NT-polyplex was injected into the ansiform lobule of the cerebellum, which contains low- but not high-affinity NT receptors. Neither internalization nor expression was observed in cultured glial cells, despite the NT-polyplex binding to those cells that was prevented by levocabastine, a low-affinity NT receptor antagonist. Conclusions. These results suggest that high-affinity NT receptors mediatethe uptake of NT-polyplex with the subsequent reporter gene expression in vivo. NT polyfection may be used to transfer genes of physiologic interest to nigrostriatal dopamine neurons, and to produce transgenic animal models of dopamine-related diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 18:24:52