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Titolo:
Stage-sensitive blockade of pituitary somatomammotrope development by targeted expression of a dominant negative epidermal growth factor receptor in transgenic mice
Autore:
Roh, M; Paterson, AJ; Asa, SL; Chin, E; Kudlow, JE;
Indirizzi:
Univ Alabama, Dept Med Endocrinol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Endocrinol, Birmingham, AL 35294 USA Univ Alabama, Dept Cell Biol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Cell Biol, Birmingham, AL 35294 USA Univ Toronto, Dept Pathol, Toronto, ON M5G 2M9, Canada Univ Toronto Toronto ON Canada M5G 2M9 athol, Toronto, ON M5G 2M9, Canada
Titolo Testata:
MOLECULAR ENDOCRINOLOGY
fascicolo: 4, volume: 15, anno: 2001,
pagine: 600 - 613
SICI:
0888-8809(200104)15:4<600:SBOPSD>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
L-TRIIODOTHYRONINE TREATMENT; RAT ANTERIOR-PITUITARY; FACTOR-ALPHA; GENE-EXPRESSION; LUTEINIZING-HORMONE; HYPOTHYROID RATS; CELL-TYPES; GONADOTROPIN-SECRETION; HOMEODOMAIN FACTOR; RATHKES-POUCH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Kudlow, JE Univ Alabama, Dept Med Endocrinol & Cell Biol, 1807 7th Ave S,Room 756, Birmingham, AL 35294 USA Univ Alabama 1807 7th Ave S,Room 756 Birmingham AL USA 35294 SA
Citazione:
M. Roh et al., "Stage-sensitive blockade of pituitary somatomammotrope development by targeted expression of a dominant negative epidermal growth factor receptor in transgenic mice", MOL ENDOCR, 15(4), 2001, pp. 600-613

Abstract

The epidermal growth factor receptor (EGFR) and its ligands EGF and transforming growth factor-alpha (TGF alpha) are expressed in the anterior pituitary, and overexpression of TGF alpha in the lactotrope cells of the pituitary gland in transgenic mice results in lactotrope hyperplasia and adenomata, suggesting a role for EGFR signaling in pituitary cell proliferation, To address the role of EGFR signaling in pituitary development in vivo, we blocked EGFR signaling in transgenic mice using the dominant negative properties of a mutant EGFR lacking an intracellular protein kinase domain (EGFR-tr), We directed EGFR-tr expression to GH- and PRL-producing cells using GH and PRL promoters, and a tetracycline-inducible gene expression system, to allow temporal control of gene expression, EGFR-tr overexpression in GH-producing cells during embryogenesis resulted in dwarf mice with pituitary hypoplasia. Both somatotrope and lactotrope development were blocked, However, when EGFR-tr overexpression was delayed to the postnatal period either by directing its expression with the PRL promoter or by delaying the onset of induction with tetracycline in the GH cells, no specific phenotype was observed. Lactotrope hyperplasia during pregnancy also occurred normally in the PRL-EGFR-tr mice. These data suggest that EGFR signaling is required for the differentiation and/or maintenance of somatomammotropes early in pituitary organogenesis but not later in life.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 22:01:10