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Titolo:
Expression and sub cellular localization of the sodium hydrogen exchanger isoform-1 in rat tissues: A possible functional relevance
Autore:
Khan, I; Thomas, N; Haridas, S;
Indirizzi:
Kuwait Univ, Fac Med, Dept Biochem, Kuwait, Kuwait Kuwait Univ Kuwait Kuwait t Univ, Fac Med, Dept Biochem, Kuwait, Kuwait
Titolo Testata:
MOLECULAR AND CELLULAR BIOCHEMISTRY
fascicolo: 1-2, volume: 219, anno: 2001,
pagine: 153 - 161
SICI:
0300-8177(200103)219:1-2<153:EASCLO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA+/H+ EXCHANGE; MESSENGER-RNA; EXPERIMENTAL COLITIS; INTRACELLULAR PH; NA/H EXCHANGER; NHE-1 ISOFORM; MUTANT MICE; TOPOLOGY; ANTIPORT; MUSCLE;
Keywords:
NHE-1; fusion-protein; apical membrane; basolateral membrane; localization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Khan, I Kuwait Univ, Fac Med, Dept Biochem, POB 24923, Kuwait, Kuwait Kuwait Univ POB 24923 Kuwait Kuwait m, POB 24923, Kuwait, Kuwait
Citazione:
I. Khan et al., "Expression and sub cellular localization of the sodium hydrogen exchanger isoform-1 in rat tissues: A possible functional relevance", MOL C BIOCH, 219(1-2), 2001, pp. 153-161

Abstract

In an attempt to understand the mechanism underlying the tissue-dependent function, the expression of NHE-1 protein and its sub cellular localizationwas examined in the rat GI-tract and other tissues. Rat NHE-1 polyclonal antibodies were raised in rabbits using a NHE-1 fusion protein antigen. The antibodies recognized a 110 kD protein in rats and mice, but not in human or rabbit RBCs. Colon, stomach, brain, spleen and kidney expressed NHE-1 protein abundantly, whereas the skeletal muscle the least abundant. Ouabain-sensitive-K+-stimulated p-nitrophenylphosphatase (PNPPase), the partial activity of the sodium pump and alkaline phosphatase (Apase) were used as the markers of the basolateral and apical membranes. NHE-1 was detected predominantly in the PNPPase enriched membrane fractions, but was also detected in the apical membrane enriched fractions in the kidney cortex, jejunum and colon at a lower level. NHE-1 was detected in the plasma membrane enriched fractions from the skeletal muscle and ventricle. Immunofluorescence data showed a similar localization pattern of NHE-1 in the colon and kidney sections. These findings suggest that NHE-1 is localized both on the apical and basolateral membrane. In view of its similar sub cellular localization in the GI-tract and kidney, but a different level of expression, might suggest that the level of protein, but not the sub cellular distribution is important to regulate its tissue-dependent function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 21:42:15