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Titolo:
Rhesus monkey model for Leishmania major transmitted by Phlebotomus papatasi sandfly bites
Autore:
Probst, RJ; Wellde, BT; Lawyer, PG; Stiteler, JS; Rowton, ED;
Indirizzi:
WRAIR, Dept Entomol, Silver Spring, MD 20910 USA WRAIR Silver Spring MD USA 20910 ept Entomol, Silver Spring, MD 20910 USA Walter Reed Army Inst Res, Div Vet Med, Silver Spring, MD 20910 USA WalterReed Army Inst Res Silver Spring MD USA 20910 Spring, MD 20910 USA Uniformed Serv Univ Hlth Sci, Dept Prevent Med & Biometr, Bethesda, MD 20814 USA Uniformed Serv Univ Hlth Sci Bethesda MD USA 20814 Bethesda, MD 20814 USA
Titolo Testata:
MEDICAL AND VETERINARY ENTOMOLOGY
fascicolo: 1, volume: 15, anno: 2001,
pagine: 12 - 21
SICI:
0269-283X(200103)15:1<12:RMMFLM>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMORAL IMMUNE-RESPONSES; CUTANEOUS LEISHMANIASIS; VERVET MONKEYS; CERCOPITHECUS-AETHIOPS; VISCERAL LEISHMANIASIS; EXPERIMENTAL-INFECTION; PARASITE TRANSMISSION; LUTZOMYIA-LONGIPALPIS; PRESBYTIS-ENTELLUS; NONHUMAN-PRIMATES;
Keywords:
Leishmania major; Macaca mulatta; Phlebotomus papatasi; cutaneous leishmaniasis; membrane feeding; monkey; primate model; rhesus macaque; parasite transmission; phlebotomine sandflies; sandfly bite;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Rowton, ED WRAIR, Dept Entomol, 503 Robert Grant Ave, Silver Spring, MD 20910 USA WRAIR 503 Robert Grant Ave Silver Spring MD USA 20910 20910 USA
Citazione:
R.J. Probst et al., "Rhesus monkey model for Leishmania major transmitted by Phlebotomus papatasi sandfly bites", MED VET ENT, 15(1), 2001, pp. 12-21

Abstract

Leishmaniasis research needs a near-human model for investigations of natural infection processes, immunological responses and evaluation of treatments. Therefore, we developed a reproducible system using Leishmania major Yakimoff & Schokhor (Trypanosomatidae: Kinetoplastida), the cause of Old World zoonotic cutaneous leishmaniasis (ZCL), transmitted to rhesus monkeys Macaca mulatta (Zimmerman) (Primates: Cercopithecidae) by sandfly bites of experimentally infected Phlebotomus papatasi (Scopoli) (Diptera: Psychodidae). Eight monkeys of presumed Indian origin (Leishmania naive) were exposed tobites of female sandflies that had been infected with L. major by membrane-feeding on human blood seeded with amastigotes isolated from hamster footpad lesions. Infection rates of membrane-fed sandflies averaged >85% seven days after the infective feed, with uniformly high numbers of promastigotes in the stomodaeal valve region of the sandfly gut. Nodules and ulcerating dermal lesions developed on 7/8 monkeys 2-4 weeks post-bite and persisted for 3-7 months. Monkeys also developed satellite lesions beyond the area of sandfly bites on the head, but not on the chest. Three rechallenged monkeys developed lesions that healed faster than lesions from their primary challenges. After infection, monkeys developed delayed type hypersensitivity (DTH) responses to a panel of Leishmania skin test antigens (LSTA) and, when tested by ELISA and IFA, showed significant post-infection antibody titres which typically rose for similar to 170 days and then gradually receded during the next 100 days following the first challenge. After the second challenge, antibody titres spiked higher within similar to 50 days and receded more rapidly. In contrast, four rhesus macaques of Chinese origin developed nolesions following infected sandfly bites, although they raised antibodies and LSTA reactions, indicating subclinical infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/02/20 alle ore 18:00:52