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Titolo:
Determination of canine beta 2-microgloblin in plasma and urine by enzyme-linked immunosorbent assay
Autore:
Nakajima, Y; Hoshi, F; Higuchi, S; Kawamura, S;
Indirizzi:
Kitasato Univ, Sch Vet Med & Anim Sci, Lab Vet Internal Med, Towada, Aomori 0348628, Japan Kitasato Univ Towada Aomori Japan 0348628 , Towada, Aomori 0348628, Japan
Titolo Testata:
JOURNAL OF VETERINARY MEDICAL SCIENCE
fascicolo: 3, volume: 63, anno: 2001,
pagine: 343 - 345
SICI:
0916-7250(200103)63:3<343:DOCB2I>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM; ELISA; BETA(2)-MICROGLOBULIN; RADIOIMMUNOASSAY; IMMUNOASSAY; SAMPLES;
Keywords:
canine; ELIS; beta(2)-microglobulin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Nakajima, Y Kitasato Univ, Sch Vet Med & Anim Sci, Lab Vet Internal Med, Towada, Aomori 0348628, Japan Kitasato Univ Towada Aomori Japan 0348628 mori 0348628, Japan
Citazione:
Y. Nakajima et al., "Determination of canine beta 2-microgloblin in plasma and urine by enzyme-linked immunosorbent assay", J VET MED S, 63(3), 2001, pp. 343-345

Abstract

An enzyme-linked immunosorbent assay was developed for the determination of canine beta (2)-microglobulin (beta (2)-m) in plasma and urine. The detectable sensitivity for pure canine beta (2)-m was 0.05 mug/l and the analytical range was 0.1 to 50 mug/l. The mean analytical recovery when pure canine beta (2)-m was added to normal plasma was 101.9%. The mean analytical recovery in the urine was 102.1%. The intra-day variation coefficient was 3.1%in plasma, 4.3% in serum and 1.9% in urine. No difference was found between the concentration of beta (2)-m in plasma and serum (n=17). The concentration of beta (2)-m in the plasma of normal dogs was 1.82 +/- 0.57 mg/l (n=31). The mean excretion in 24 hr urine collected from normal dogs was 17.6 +/- 9.2 mug/l; 0.22 +/- 0.12. mug/kg of body weight or 14.2 +/- 9.4 mug/g ofurine creatinine. The beta (2)-m creatinine index of random urine samples was 23.5 +/- 16.6 mug/kg (n=26). There was a close correlation between the beta (2)-m creatinine index of 24 hr urine samples and that of random urinesamples (r=0.872).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:08:02