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Titolo:
Thrombospondin-1 is the key activator of TGF-beta 1 in human mesangial cells exposed to high glucose
Autore:
Yevdokimova, N; Wahab, NA; Mason, RM;
Indirizzi:
Univ London Imperial Coll Sci Technol & Med, Sch Med, Div Biomed Sci, Mol Pathol Sect, London SW7 2AZ, England Univ London Imperial Coll Sci Technol & Med London England SW7 2AZ gland
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
fascicolo: 4, volume: 12, anno: 2001,
pagine: 703 - 712
SICI:
1046-6673(200104)12:4<703:TITKAO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; TGF-BETA; TRANSFORMING GROWTH-FACTOR-BETA-1; DIABETES-MELLITUS; MESSENGER-RNA; IN-VIVO; EXPRESSION; FIBRONECTIN; FIBROSIS; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Mason, RM Univ London Imperial Coll Sci Technol & Med, Sch Med, Div BiomedSci, Mol Pathol Sect, Sir Alexander Fleming Bldg,Exhibit Rd, London SW7 2AZ, England Univ London Imperial Coll Sci Technol & Med Sir Alexander Fleming Bldg,Exhibit Rd London England SW7 2AZ
Citazione:
N. Yevdokimova et al., "Thrombospondin-1 is the key activator of TGF-beta 1 in human mesangial cells exposed to high glucose", J AM S NEPH, 12(4), 2001, pp. 703-712

Abstract

Elevated levels of transforming growth factor-beta1 (TGF-beta1) are synthesized by human mesangial cells that are cultured in medium that contains high concentrations of glucose and mediate increased synthesis of fibronectin(FN), plasminogen activator inhibitor-1 (PAI-1), and changes in the expression of other genes. TGF-beta1 is synthesized as a latent complex. Previouswork indicated that high-glucose conditions also upregulate expression of thrombospondin-1 (TSP-1), a potential activator of latent TGF-beta1. With the use of the synthetic peptide GGWSHW, an inhibitor of the TSP-1 activation mechanism, endogenous TSP-1 is shown to be responsible for converting high levels of latent TGF-beta1 to bioactive growth factor over 3 wk of exposure of mesangial cells to 30 mM D-glucose. Peptide inhibition of TGF-beta1 activation by TSP-1 in high-glucose conditions completely suppressed increases in FN and PAI-1 expression. Treating mesangial cells maintained in high glucose with a TSP-1 antisense oligonucleotide reduced TSP-1 expression to levels found in 4 mM D-glucose cultures, prevented TGF-beta1 activation, and normalized expression of FN.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 05:45:02