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Titolo:
THE APPLICABILITY OF FORMALIN-FIXED AND FORMALIN-FIXED PARAFFIN-EMBEDDED TISSUES IN FORENSIC DNA ANALYSIS
Autore:
ROMERO RL; JUSTON AC; BALLANTYNE J; HENRY BE;
Indirizzi:
WASHOE CTY SHERIFFS OFF,FORENS SCI DIV,911 PARR BLVD RENO NV 89512 SUFFOLK CTY CRIME LAB HAUPPAUGE NY 00000 ALABAMA DEPT FORENS SCI BIRMINGHAM AL 00000
Titolo Testata:
Journal of forensic sciences
fascicolo: 4, volume: 42, anno: 1997,
pagine: 708 - 714
SICI:
0022-1198(1997)42:4<708:TAOFAF>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
TANDEM REPEAT LOCI; EMPLOYING MULTIPLEX AMPLIFICATION; POLYMERASE CHAIN-REACTION; PCR-AMPLIFICATION; VNTR LOCUS; STR LOCI; SEQUENCES; IDENTIFICATION; SAMPLES; SYSTEM;
Keywords:
FORENSIC SCIENCE; DNA TYPING; POLYMERASE CHAIN REACTION; FORMALIN; TISSUE FIXATION; HLA-DQ; LDLR; GYPA; HBGG; D7S8; GC; SHORT TANDEM REPEATS; VWA31; THO1; F13A1; FES/FPS; TPOX; CSF1PO; D1S80;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
R.L. Romero et al., "THE APPLICABILITY OF FORMALIN-FIXED AND FORMALIN-FIXED PARAFFIN-EMBEDDED TISSUES IN FORENSIC DNA ANALYSIS", Journal of forensic sciences, 42(4), 1997, pp. 708-714

Abstract

Historically, formalin fixed (FF) tissues could not be used as a source of DNA in forensic science due to the fact that the DNA was too degraded for DNA analysis. With the introduction of the polymerase chain reaction (PCR) technique to forensic science, the usefulness of DNA from this biological material has been re-evaluated. This study evaluates the potential use of DNA from FF and formalin fixed paraffin embedded (FFPE) tissues in 13 PCR systems; HLA DQ alpha, LDLR, GYPA, HBGG, D7S8, GC, D1S80, vWA31, THO1, F13A1, FES/FPS, TPOX, and CSF1PO. The first six, HLA DQ alpha, LDLR, GYPA, HBGG, D7S8, and GC are reverse dot blot systems, D1S80 is an amplified fragment length polymorphism (AmpFlp) system and the others are short tandem repeats (STRs). This study shows that FFPE tissue which has not been fixed in formalin for more than three days is a useful source of DNA for 12 of the 13 PCR systems. Ln contrast, FP:tissue did not prove to be a reliable source of DNA forthe PCR techniques examined here.

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Documento generato il 30/11/20 alle ore 20:07:05