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Titolo:
A new HPLC micromethod to measure total plasma homocysteine in newborn
Autore:
Bartesaghi, S; Accinni, R; De Leo, G; Cursano, CF; Campolo, J; Galluzzo, C; Vegezzi, PG; Parodi, O;
Indirizzi:
Sacra Famiglia FBF AfaR, Erba, CO, Italy Sacra Famiglia FBF AfaR Erba CO Italy Famiglia FBF AfaR, Erba, CO, Italy Niguarda Hosp, CNR, Inst Clin Physiol, Sect Milan, Milan, Italy Niguarda Hosp Milan Italy , Inst Clin Physiol, Sect Milan, Milan, Italy
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 5-6, volume: 24, anno: 2001,
pagine: 1137 - 1141
SICI:
0731-7085(200103)24:5-6<1137:ANHMTM>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE DETECTION; PEDIATRIC-PATIENTS; HYPERHOMOCYSTEINEMIA; CHROMATOGRAPHY; METHIONINE; CYSTEINE; SERUM; ASSAY; URINE;
Keywords:
total plasma homocysteine; newborn; high-performance liquid chromatography;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Bartesaghi, S Sacra Famiglia FBF AfaR, Erba, CO, Italy Sacra Famiglia FBF AfaR Erba CO Italy faR, Erba, CO, Italy
Citazione:
S. Bartesaghi et al., "A new HPLC micromethod to measure total plasma homocysteine in newborn", J PHARM B, 24(5-6), 2001, pp. 1137-1141

Abstract

Total plasma homocysteine (tHcy) in children may be an useful biochemical marker for genetic risk of premature cardiovascular disease. We reported a rapid, isocratic HPLC method able to process very small amount of newborn plasma samples. A blood sample from heel capillary circulation was collected, using a heparinized capillary glass tube. Plasma sample from 1 to 10 mul was derivatized with ammonium-7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate after reduction with tri-n-butylphosphine and analyzed on Discovery C18 column, with a solution of acetonitrile-dihydrogenphosphate 0.1 M (8:92 v/v pH*2.1). This assay ensures a good recovery (95%), precision (CV 4.5%) and linearity (y = 2.41x + 0.31, r = 1). Due to its simplicity and reliability, our method is suitable for routine analysis of tHcy and other aminothiols (Cys, Cys-Gly, GSH) assessed for clinical and research purposes. With this HPLC method we have assayed tHcy levels in 1400 apparently healthy newborn babies (tHcy mean value = 4.9 +/- 2.7 muM). In conclusion, this accurate and linear HPLC method allows measurement of tHcy in newborn during the routinary capillary blood collection in the fourth living day without any other invasive procedure. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 12:04:11