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Titolo:
Doping control for nandrolone using hair analysis
Autore:
Kintz, P; Cirimele, V; Dumestre-Toulet, V; Ludes, B;
Indirizzi:
Univ Strasbourg 1, Inst Med Legale, F-67000 Strasbourg, France Univ Strasbourg 1 Strasbourg France F-67000 , F-67000 Strasbourg, France Lab BIOffice, F-33370 Artigues Pres Bordeaux, France Lab BIOffice Artigues Pres Bordeaux France F-33370 Pres Bordeaux, France
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 5-6, volume: 24, anno: 2001,
pagine: 1125 - 1130
SICI:
0731-7085(200103)24:5-6<1125:DCFNUH>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANABOLIC-STEROIDS; AGENTS; 19-NORANDROSTERONE; ESTERS;
Keywords:
nandrolone; doping; hair; abuse; steroid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Kintz, P Univ Strasbourg 1, Inst Med Legale, 11 Rue Humann, F-67000 Strasbourg, France Univ Strasbourg 1 11 Rue Humann Strasbourg France F-67000 France
Citazione:
P. Kintz et al., "Doping control for nandrolone using hair analysis", J PHARM B, 24(5-6), 2001, pp. 1125-1130

Abstract

A sensitive, specific and reproducible method For the quantitative determination of nandrolone in human hair has been developed. The sample preparation involved a decontamination step of the hair with methylene chloride. Thehair sample (about 100 mg) was solubilized in 1 ml NaOH IN, 15 min at 95 degreesC, in presence of 10 ng nandrolone-d(3) used as an internal standard. The homogenate was neutralized and extracted using consecutively a solid phase (Isolute C18) and a liquid liquid (pentane) extraction. The residue was derivatized by adding 50 mul MSTFA/NH4I/2-mercaptoethanol (1000:2:5; v/v/v), then incubated for 20 min at 60 degreesC. A 4-mul aliquot of the derivatized extract was injected into the column (HP5-MS capillary column, 5%, phenyl-95% methylsiloxane, 30 m x 0.25 mm i.d. x 0.25 mm film thickness) of aHewlett Packard (Palo Alto, CA) gas chromatograph (6890 Series) via a Hewlett Packard (7673) autosampler. The assay was capable of detecting 0.5 pg of nandrolone per mg of hair when approximately 100 mg of hail were processed. Linearity was observed for nandrolone concentrations ranging from 1 to 50 pg/mg with a correlation coefficient of 0.997. Intra-day and between-day precisions at 10 pg;mg were 11.2 and 15.1%, respectively. with an extraction recovery of 81.7%. The analysis of three strands of hair, obtained from three bodybuilders, revealed the presence of nandrolone at the concentrationof 1, 3.5 and 7.5 pg/mg. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 04:33:45