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Titolo:
A novel bovine lactoferrin peptide, FKCRRWQWRM, suppresses Candida cell growth and activates neutrophils
Autore:
Ueta, E; Tanida, T; Osaki, T;
Indirizzi:
Kochi Med Sch, Dept Oral Surg, Nankoku, Kochi 7838505, Japan Kochi Med Sch Nankoku Kochi Japan 7838505 , Nankoku, Kochi 7838505, Japan
Titolo Testata:
JOURNAL OF PEPTIDE RESEARCH
fascicolo: 3, volume: 57, anno: 2001,
pagine: 240 - 249
SICI:
1397-002X(200103)57:3<240:ANBLPF>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMORPHONUCLEAR LEUKOCYTES; ALBICANS INFECTION; ANTIFUNGAL AGENTS; FUNGAL-INFECTIONS; IN-VITRO; MICE; SALIVA; MILK; EPIDEMIOLOGY; TRANSFERRIN;
Keywords:
Candida albicans; Candida killing; FKCRRWQWRM; lactoferrin peptides; neutrophils;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Ueta, E Kochi Med Sch, Dept Oral Surg, Nankoku, Kochi 7838505, Japan KochiMed Sch Nankoku Kochi Japan 7838505 u, Kochi 7838505, Japan
Citazione:
E. Ueta et al., "A novel bovine lactoferrin peptide, FKCRRWQWRM, suppresses Candida cell growth and activates neutrophils", J PEPT RES, 57(3), 2001, pp. 240-249

Abstract

To identify potent new antifungal agents, the Candida cell growth inhibitory activities of six lactoferrin (Lf) peptides consisting of 6-25 amino acid residues (peptide 1, FKCRRWQWRMKKLGAPSITCVRRAF = lactoferricin B; peptide2, FKCRRWQWRM; peptide 2', FKARRWQWRM; peptide 3, GAPSITCVRRAF; peptide 4,RRWQWR; and peptide 5, RWQWRM) were examined. Of these, peptide 2 stronglysuppressed the multiplication of Candida cells, but other peptides showed only weak activities. In two strains of C. albicans, the minimum inhibitoryconcentration 100 of peptide Z (17.3 +/-2.2 muM and 17.5 +/-2.4 muM) was close to that of miconazole (13.0 +/-1.7 muM and 13.1 +/-1.61 muM) but markedly different from that of amphotericin B (0.52 +/-0.09 muM and 0.56 +/-0.11 muM). The suppression of Candida cell growth was additively increased by a combination of peptide 2 with amphotericin B and miconazole. Peptides 1, 3, 4 and 5 and Lf suppressed iron uptake by Candida cells, inversely correlated with their Candida cell growth inhibition activities. However, iron uptake was not inhibited by peptide 2. In addition, peptide Z upregulated Candida cell killing activity of polymorphonuclear leukocytes (PMN) increasingtheir superoxide generation, protein kinase C activity, p38 MAPK activity and the expression of p47(phox). These results indicated that the main antimicrobial activity of the if peptides is dependent on the N-terminal half of Lf and that the PMN upregulatory activity of peptide Z and additive function of peptide Z with antifungal drugs are useful for prophylaxis and control of candidiasis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 17:55:30