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Titolo:
Partial, graded losses of dopamine terminals in the rat caudate-putamen: an animal model for the study of compensatory adaptation in preclinical parkinsonism
Autore:
Bergstrom, BP; Schertz, KE; Weirick, T; Nafziger, B; Takacs, SA; Lopes, KO; Massa, KJ; Walker, QD; Garris, PA;
Indirizzi:
Illinois State Univ, Dept Biol Sci, Cellular & Integrat Physiol Sect, Normal, IL 61790 USA Illinois State Univ Normal IL USA 61790 hysiol Sect, Normal, IL 61790 USA Duke Univ, Med Ctr, Dept Pharmacol, Durham, NC 27710 USA Duke Univ DurhamNC USA 27710 d Ctr, Dept Pharmacol, Durham, NC 27710 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 1, volume: 106, anno: 2001,
pagine: 15 - 28
SICI:
0165-0270(20010330)106:1<15:PGLODT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
EVOKED EXTRACELLULAR DOPAMINE; NIGROSTRIATAL BUNDLE DAMAGE; IN-VIVO; STRIATAL DOPAMINE; ROTATIONAL BEHAVIOR; NUCLEUS-ACCUMBENS; ELECTRICAL-STIMULATION; SUBSTANTIA-NIGRA; PARTIAL LESIONS; CYCLIC VOLTAMMETRY;
Keywords:
Parkinson's disease; 6-OHDA; dopamine; voltammetry; ventral mesencephalon; caudate-putamen; electrical stimulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Garris, PA Illinois State Univ, Dept Biol Sci, Cellular & Integrat PhysiolSect, Normal, IL 61790 USA Illinois State Univ Normal IL USA 61790 , Normal, IL 61790 USA
Citazione:
B.P. Bergstrom et al., "Partial, graded losses of dopamine terminals in the rat caudate-putamen: an animal model for the study of compensatory adaptation in preclinical parkinsonism", J NEUROSC M, 106(1), 2001, pp. 15-28

Abstract

Procedures to lesion dopamine (DA) neurons innervating the rat caudate-putamen (CP) in a partial, graded fashion are described in this study. The goal is to provide a lesion model that supports intra-animal comparisons of voltammetric recordings used to investigate compensatory adaptation of DA neurotransmission. Lesions exploited the topography of mesostriatal DA neurons, microinjections of the neurotoxin 6-hydroxydopamine (6-OHDA) into the medial and lateral edges of the ventral mesencephalon containing DA cell bodies and microdissection of the CP into six regions. Analysis of tissue DA content in these regions by HPLC-EC demonstrated that 6-OHDA injected into thelateral substantia nigra results in a significantly greater loss of DA in lateral versus medial regions of the CP. The direction of the graded loss of DA was reversed (i.e. a medial to lateral lesion gradient) by the injection of 6-OHDA into the ventral tegmental area near the medial SN. Extracellular concentrations of electrically evoked DA could be measured across the mediolateral axis of the CP in a single animal using the technique of in vivo voltammetry. More importantly, graded decreases in the amplitude of evoked DA levels generally followed the direction of the tissue DA gradient in lesioned animals. These results suggest that the graded loss of DA terminalsin the CP, coupled to a spatially and temporally resolved technique for monitoring extracellular DA, is a viable tool for investigating compensatory adaptation in the mesostriatal DA system. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 28/02/20 alle ore 13:51:53