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Titolo:
Protein and mRNA levels of the myosin heavy chain isoforms I beta, IIa, IIx and IIb in Type I and Type II fibre-predominant rat skeletal muscles in response to chronic alcohol feeding
Autore:
Reilly, ME; McKoy, G; Mantle, D; Peters, TJ; Goldspink, G; Preedy, VR;
Indirizzi:
Univ London Kings Coll, Dept Nutr & Dietet, Mol & Cellular Prot Biochem Lab, London SE1 9NN, England Univ London Kings Coll London England SE1 9NN b, London SE1 9NN, England Royal Free Hosp, Dept Dev Anat, London NW3 2QG, England Royal Free Hosp London England NW3 2QG Dev Anat, London NW3 2QG, England Newcastle Gen Hosp, Reg Neurosci Ctr, Dept Neurochem, Newcastle Upon Tyne NE4 6BE, Tyne & Wear, England Newcastle Gen Hosp Newcastle Upon Tyne Tyne & Wear England NE4 6BE ngland Univ London Kings Coll, Sch Med, Dept Clin Biochem, London SE5 9PJ, England Univ London Kings Coll London England SE5 9PJ m, London SE5 9PJ, England
Titolo Testata:
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
fascicolo: 8, volume: 21, anno: 2000,
pagine: 763 - 773
SICI:
0142-4319(200011)21:8<763:PAMLOT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC ETHANOL-CONSUMPTION; FIBER-TYPE COMPOSITION; GENE-EXPRESSION; GASTROCNEMIUS-MUSCLE; SOLEUS MUSCLE; RNA-CONTENT; MYOPATHY; DISEASE; FRACTIONS; LIVER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Preedy, VR Univ London Kings Coll, Dept Nutr & Dietet, Mol & Cellular ProtBiochem Lab, 150 Stamford St, London SE1 9NN, England Univ London Kings Coll 150 Stamford St London England SE1 9NN
Citazione:
M.E. Reilly et al., "Protein and mRNA levels of the myosin heavy chain isoforms I beta, IIa, IIx and IIb in Type I and Type II fibre-predominant rat skeletal muscles in response to chronic alcohol feeding", J MUSCLE R, 21(8), 2000, pp. 763-773

Abstract

Alcoholic myopathy occurs in between one and two-thirds of all alcohol misusers and is thus one of the most prevalent muscle disorders (2000 cases per 100,000 population). It is characterised by myalgia, muscle weakness and loss of lean tissue mass. Histological features include a reduction in the diameter of Type II muscle fibres, particularly the IIb fibre subset. In contrast, Type I fibres are relatively protected. It is possible that the myopathy is due to perturbations in myosin protein and mRNA expression. To test this hypothesis, we fed rats a liquid diet containing 35% of calories as ethanol. Control rats were pair-fed identical amounts of the same diet in which ethanol was replaced by isocaloric glucose. At the end of 6 weeks, total myofibrillary proteins and myosin heavy chain (MyoHC) I beta, IIa, IIx and IIb protein and mRNA were analysed in the plantaris (Type II fibre-predominant) and soleus (Type I fibre-predominant) muscles. The data showed thatthere were significant reductions in the total myofibrillary protein content in the plantaris of ethanol fed rats compared to pair-fed controls (P < 0.05). These changes in the plantaris were accompanied by reductions in total myosin (P < 0.025), as a consequence of specific reductions in the I beta, (P < 0.01), IIx (P < 0.05) and IIb (P < 0.05) protein isoforms. The mRNAlevels of I beta were significantly reduced in the plantaris (P < 0.05). However, mRNA levels of IIa, IIx and IIb in the plantaris were not significantly affected by alcohol feeding. Other changes in the plantaris included significant reductions in desmin (P < 0.01), actin (P < 0.025), and troponin-I (P < 0.05) compared to pair-fed controls. In the soleus, the only significant changes related to a fall in I beta mRNA levels and a decline in troponin-C content. We conclude that in the rat, alcoholic myopathy is a feature of Type II fibre rich muscles and is accompanied by multiple protein changes. The decline in specific myosin protein levels, such as IIx and IIb in the absence of corresponding reductions in their mRNAs, is probably due to altered proteolysis or more likely reductions in translational efficiencies, rather than changes in transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 12:16:53