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Titolo:
Sequence information, distinction and quantitation of C-terminal leucine and isoleucine in ternary complexes of tripeptides with Cu(II) and 2,2-' bipyridine
Autore:
Vaisar, T; Gatlin, CL; Rao, RD; Seymour, JL; Turecek, F;
Indirizzi:
Univ Washington, Dept Chem, Seattle, WA 98195 USA Univ Washington SeattleWA USA 98195 on, Dept Chem, Seattle, WA 98195 USA Molecumet Inc, Bellevue, WA 98005 USA Molecumet Inc Bellevue WA USA 98005Molecumet Inc, Bellevue, WA 98005 USA
Titolo Testata:
JOURNAL OF MASS SPECTROMETRY
fascicolo: 3, volume: 36, anno: 2001,
pagine: 306 - 316
SICI:
1076-5174(200103)36:3<306:SIDAQO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
TANDEM MASS-SPECTROMETRY; CYSTEINE-CONTAINING PEPTIDES; QUADRUPOLE ION-TRAP; GAS-PHASE COMPLEXES; ELECTROSPRAY-IONIZATION; DIIMINE LIGANDS; METAL-IONS; AMINO-ACIDS; QUINOLONE ANTIBIOTICS; ARGENTINATED PEPTIDES;
Keywords:
leucine; isoleucine; tripeptide complexes; Cu(II); 2,2 '-bipyridine; self-assembly; electrospray ionization; collision-induced dissociation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Turecek, F Univ Washington, Dept Chem, Bagley Hall,Box 351700, Seattle, WA98195 USA Univ Washington Bagley Hall,Box 351700 Seattle WA USA 98195 USA
Citazione:
T. Vaisar et al., "Sequence information, distinction and quantitation of C-terminal leucine and isoleucine in ternary complexes of tripeptides with Cu(II) and 2,2-' bipyridine", J MASS SPEC, 36(3), 2001, pp. 306-316

Abstract

Tripeptides form ternary complexes with Cu2+ and 2,2 ' -bipyridine (bpy) that self-assemble upon mixing the components in aqueous methanol solution. Electrospray ionization (ESI) of the complex solutions provides abundant singly charged [Cu(peptide - H)bpy](+) and doubly charged [Cu(peptide)bpy](2+) ions. Collision-induced dissociation (CID) at low ion kinetic energies ofseveral tripeptides, AGG, GGA, LGG, GGL, GGI, FGG, GGF, LGF, GLF, GFL, GYAand GAY, showed fragments that were indicative of the amino acid sequence in the peptide. In addition, CID of single and doubly charged complexes of isomeric tripeptides GGL and GGI provided unambiguous distinction of the isomeric leucine and isoleucine residues. Leucine peptides eliminated C3H7 radicals from the amino acid side-chain whereas isoleucine eliminated C2H5 radicals. CID of gas-phase doubly charged peptide complexes in a quadrupole ion trap produced a series of singly charged sequence fragments that following isolation and further CID furnished distinct fragments that allowed quantitation of leucine and isoleucine-containing peptides in mixtures. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 19:03:58