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Titolo:
Identification and comparative analysis of human colonocyte short-chain fatty acid response genes
Autore:
Basson, MD; Liu, YW; Hanly, AM; Emenaker, NJ; Shenoy, SG; Rothberg, BEG;
Indirizzi:
Yale Univ, Sch Med, Dept Surg, New Haven, CT 06520 USA Yale Univ New Haven CT USA 06520 Med, Dept Surg, New Haven, CT 06520 USA Connecticut VA Hlth Care Syst, Surg Serv, W Haven, CT USA Connecticut VA Hlth Care Syst W Haven CT USA Surg Serv, W Haven, CT USA CuraGen Corp, New Haven, CT USA CuraGen Corp New Haven CT USACuraGen Corp, New Haven, CT USA
Titolo Testata:
JOURNAL OF GASTROINTESTINAL SURGERY
fascicolo: 5, volume: 4, anno: 2000,
pagine: 501 - 512
SICI:
1091-255X(200009/10)4:5<501:IACAOH>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITHELIAL-CELL PROLIFERATION; PRECURSOR-LIKE PROTEIN-2; EPIDERMAL GROWTH-FACTOR; SODIUM-BUTYRATE; HISTONE ACETYLATION; MESSENGER-RNA; HOMEOBOX GENE; APOLIPOPROTEIN-J; DIETARY FIBER; CDX2 HOMEOBOX;
Keywords:
acetate; butyrate; Caco-2; colon cancer; gene expression; propionate; short-chain fatty acids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Basson, MD Yale Univ, Sch Med, Dept Surg, 333 Cedar St,POB 208062, New Haven, CT 06520 USA Yale Univ 333 Cedar St,POB 208062 New Haven CT USA 06520 20 USA
Citazione:
M.D. Basson et al., "Identification and comparative analysis of human colonocyte short-chain fatty acid response genes", J GASTRO S, 4(5), 2000, pp. 501-512

Abstract

Short-chain fatty acids (SCFAs) butyrate, propionate, and acetate producedduring fiber fermentation promote colonic differentiation and can reverse or suppress neoplastic progression. We sought to identify candidate genes responsible for SCFA activity on colonocytes and to compare the relative activities of independent SCFAs. cDNA was generated from polyA(+) mRNA isolated from control Caco-2 cells and cells treated with equimolar butyrate, propionate, and acetate. GeneCalling, a restriction-based differential mRNA expression platform linked to a DNA sequence database lookup, was applied. A total of 30,000 individual genetic sequences were analzyed for differential expression among the three SCFAs. Differentially expressed peaks corresponding to cancer-related genes were isolated, sequenced, and cross-referenced to the GenBank human database. Gene identities were independently confirmedby oligonucleotide poisoning. More than 1000 gene fragments were identified as being substantially modulated in expression by butyrate. Butyrate tended to have the most pronounced effects and acetate the least. Five fragments selected for further study were fully sequenced and proved 100% homologous with human sequences for clusterin, amyloid precursor-like protein 2, andcaudal homeobox 2 protein, not previously known to be modulated by SCFAs. In each case, a similar order of potency for the three SCFAs studied was observed. The common SCFAs appear to exert different effects. This study suggests the diversity of the SCFA response at the molecular level and facilitates identifying genes important in the biologic activity of dietary fiber.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 05:49:44